Skip to main content

Semi-automated histogram analysis of normal bone marrow using 18F-FDG PET/CT: correlation with clinical indicators

Abstract

Background

18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) is increasingly applied to the diagnosis of bone marrow failure such as myeloproliferative neoplasm, aplastic anemia, and myelodysplastic syndrome, as well as malignant lymphoma and multiple myeloma. However, few studies have shown a normal FDG uptake pattern. This study aimed to establish a standard of bone marrow FDG uptake by a reproducible quantitative method with fewer steps using deep learning-based organ segmentation.

Methods

Bone marrow PET images were obtained using segmented whole-spine and pelvic bone marrow cavity CT as mask images using a commercially available imaging workstation that implemented an automatic organ segmentation algorithm based on deep learning. The correlation between clinical indicators and quantitative PET parameters, including histogram features, was evaluated.

Results

A total of 98 healthy adults were analyzed. The volume of bone marrow PET extracted in men was significantly higher than that in women (p < 0.0001). Univariate and multivariate regression analyses showed that mean of standardized uptake value corrected by lean body mass (SULmean) and entropy in both men and women were inversely correlated with age (all p < 0.0001), and SULmax in women were also inversely correlated with age (p = 0.011).

Conclusion

A normal FDG uptake pattern was demonstrated by simplified FDG PET/CT bone marrow quantification.

Peer Review reports

Background

Whole-body 18F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) is a glucose metabolism-based imaging technique that is currently used for staging, restaging, and therapeutic evaluation of malignant tumors. PET is a diagnostic imaging modality with excellent quantitative properties and the various quantitative values that are calculated based on the standardized uptake value (SUV), which is a semi-quantitative value of FDG accumulation, are useful for distinguishing benign and malignant tumors and predicting their prognosis [1,2,3].

In addition to malignant tumors, bone marrow (BM) FDG uptake could be affected by hematologic, immune, and inflammatory disorders, which cause increased cellularity and hyperactivity in the BM [4,5,6,7]. BM FDG uptake has also been reportedly associated with white blood cell (WBC) count, serum C-reactive protein, and granulocyte-colony stimulating factor or erythropoietin [8, 9]. Although BM biopsy remains the gold standard for BM pathology diagnosis in clinical practice, BM biopsies are highly invasive and contain many sampling errors. Particular attention should be paid to the irregular distribution of local areas of active bone marrow and/or hematological malignancies, and thus, the lesions may be missed on random biopsies.

FDG-PET/CT has enabled less invasive visualization of glucose metabolism in BM throughout the body, which was considerably difficult with conventional morphological imaging such as CT and magnetic resonance imaging [10]. Recently, FDG PET/CT has been increasingly applied to the diagnosis of bone marrow failure such as myeloproliferative neoplasm, aplastic anemia, and myelodysplastic syndrome as well as malignant lymphoma and multiple myeloma [11,12,13,14]. Some studies have reported that the region-of-interest was manually placed on certain bones and based on the semi-automatic measurement method for quantitative evaluation of BM [14, 15]. The segmentation of whole BM by a computational approach of PET/CT images has also been reported [16, 17]. However, the PET quantification method for BM, which was proposed in the previous studies, was complicated and has not been clinically applied. In addition, the quantitative values representing the FDG uptake pattern of normal bone marrow as a baseline have been undefined.

Therefore, the purpose of this study was to establish a standard for bone marrow FDG uptake patterns by a reproducible quantitative method with fewer steps using a commercially available automated image analysis software.

Methods

Participant enrollment

Between May 2017 and March 2020, 667 consecutively registered participants underwent whole-body PET/CT for cancer screening at our institution. The inclusion criteria were: (i) adults aged 20–80 years, (ii) no abnormalities detected on PET/CT, and absence of abnormalities confirmed on subsequent PET/CT within the following two years, and (iii) answered a questionnaire about medical history, abnormal symptoms, sex, age, and smoking history before cancer screening. Participants with a history of malignancies, systemic diseases (hematologic disorders, immune diseases, infection/inflammation, fever), and those receiving hormonal therapy, chemotherapy, immunosuppressive therapy, or steroids were excluded. Participants with diabetes and those with blood glucose levels ≥ 150 mg/dL before FDG injection were also excluded. However, there were no adults in their 20s who underwent PET for the purpose of cancer screening. It has been considered better to include those in their 20s in order to target "healthy adults". For that reason, four women in their 20s who had a PET scan for purposes other than health checks and were finally diagnosed as "healthy" were added. Unfortunately, no man in his twenties met these inclusion criteria.

Participants were excluded from this study if they had white blood cell (WBC) count, mean corpuscular volume, hemoglobin level, platelets count, lactic acid dehydrogenase, creatinine, aspartate aminotransferase, alanine aminotransferase, and total bilirubin levels in their blood data that are not in the normal range (Table 1). No participant had splenomegaly on CT [18]. All the participants were considered healthy without clinical suspicion of malignancy or a severe inflammatory condition based on the diagnosis by experienced physicians who performed medical interviews and physical examinations, and imaging diagnostic radiologists who diagnosed them comprehensively.

Table 1 Blood data items referenced for the definition of healthy adults in this study

This single-institution study was approved by our institutional review board and was conducted in accordance with the Declaration of Helsinki. Owing to the retrospective study design and use of anonymized patient data, the requirement for informed consent was waived. We had the right to access the clinical/personal patient data used in this study and were not required to obtain any special administrative permissions or licenses.

Imaging protocol

All the patients fasted for at least 6 h before 18F-FDG (3 MBq/kg) administration. Whole-body PET/CT scans were performed at 60-min post injection using a Biograph Horizon TrueV FDG-PET/CT system (Siemens Medical Solutions, Knoxville, TN, USA). The coincidence-timing window and time-of-flight system-timing resolution were 4.1 ns and 540 ps, respectively. A CT scan was performed for attenuation correction (tube voltage, 130 kV; tube current, 15–70 mA; tube rotation time, 0.6 s per rotation; pitch, 1; transaxial field of view [FOV], 700 mm; and section thickness, 5 mm). The PET/CT images were reconstructed using the ordered subset expectation–maximization method and time-of-flight algorithm with 4 iterations and 10 subsets. The CT data were resized from a 512 × 512 matrix to a 180 × 180 matrix to match the PET data and construct the CT-based transmission maps for attenuation correction of the PET data with a post-reconstruction smoothing Gaussian filter (5-mm FWHM). The reconstructed voxel size of the PET/CT images was 4.11 × 4.11 × 5 mm.

Preparation of BM PET

The extraction of “BM PET” was planned in two major steps using a CT volume analyzer (SYNAPSE VINCENT medical imaging system; Fujifilm Medical, Tokyo, Japan) which implemented an algorithm for automatic bone segmentation based on deep learning [19, 20]. In order to run this image analyzer, it was necessary to install it on a computer with an operating system of Windows 7 or later versions, a Pentium 4 or more central processing unit, and memory of 1 GB or more.

Figure 1 shows an overview of the process. In this study, the spine and pelvic bone marrows, which are the main components of hematopoiesis in disease-free adults, were segmented [15, 21]. The extremities were excluded in this study since these PET/CTs were performed for cancer screening in healthy adults, and skull was also excluded since it was affected by artifacts due to the high physiological FDG uptake of the brain. To summarize the "BM CT" segmentation procedure, first, on CT, all spinal bones were segmented using the automatic organ extraction algorithm for "whole spine" implemented in the volume analyzer. Separately, the pelvic bone was segmented using an algorithm for extracting certain designated bones and then added to the previously obtained total spinal bones. In addition, 2 pixels were removed from the margin to exclude the bone cortex. When extraosseous tissue was extracted due to a high CT value, metal, thyroid gland, among others, the relevant part was manually removed (Fig. 2). Second, "BM PET" was extracted from whole-body PET images using "BM CT" as a mask image. BM of the 11th thoracic vertebra (Th11) was extracted separately from the BM of the whole spine and pelvis with the same procedure for Deauville 5-point scaling.

Fig. 1
figure 1

Two major procedures to obtain bone marrow (BM) positron emission tomography (PET). First: “BM computed tomography (CT)" was obtained. Whole spine and pelvic bones, including the BM, were automatically extracted from whole-body CT images using a bone-extraction algorithm implemented in the analyzer for automatic bone segmentation based on multi-task three-dimensional fully convolutional networks (3D-FCN) [19]. A multi-task 3D-FCN for organ segmentation has surpassed the two-dimensional FCN approach [20] by using data from multiple segmentation datasets and showed improvement over the single-task 3D-FCN approach (a). The contour lines were subsequently set to 2 pixels inside the first line, which were able to extract the BM and remove as much cortical bone as possible (b). Second: overlay bone marrow CT on whole-body PET (c), and obtain “bone marrow PET” masked with bone marrow CT (d)

Fig. 2
figure 2

Addition of manual removal of the extracted extramedullary region due to high CT value, thyroid gland (a), metal for dental treatment (b). Images of a case where the pelvic muscles extracted continuously with the pelvic bones were manually removed (c). No obvious artifacts were visible

A board-certified nuclear medicine physician performed a series of image processing procedures. To assess reproducibility, the same interpreter repeated these steps twice for all participants.

Data analysis

SUV is often used as a PET parameter for the evaluation of solid tumors; however, the SUV corrected by lean body mass, referred to as SUL, was used in this study for an accurate evaluation of normal bone marrow with large volumes. This is because FDG was injected in proportion to the body weight, and although the body weight depends on fat, the uptake of FDG in fat is small. Lean body weight was defined according to a previous study [22].

After converting all voxel values in the BM PET image to SULs, the quantitative PET parameters of maximum SUV corrected by lean body mass (LBM) (SULmax), mean of SUL (SULmean), and entropy, one of the features of the grayscale histogram, were calculated.

The SUL and LBM of a given tissue were calculated using the following formula [22]:

$$\begin{aligned} & {\text{SUL}} = \frac{{{\text{Tumor}}\;{\text{activity}}\;{\text{concentration }}\left( {{\text{Bq}}/{\text{ml}}} \right)}}{{{\text{Injected}}\;{\text{dose }}\left( {{\text{Bq}}} \right)}} \times {\text{LBM}}\;\left( {\text{g}} \right), \\ & {\text{LBM}}^{{{\text{man}}}} = 9270 \times \frac{{{\text{Body}}\;{\text{weight}}}}{{6680 + 216{ } \times {\text{ BMI}}}} , \\ & {\text{LBM}}^{{{\text{woman}}}} = 9270 \times \frac{{\text{Body weight}}}{{8780 + 244{ } \times {\text{ BMI}}}} . \\ \end{aligned}$$

The SULmean is the average value of extracted BM PET images.

In addition, the liver and the mediastinal SULs were measured using syngo.via VB10 software (Siemens Healthcare GmbH, Erlangen, Germany), which implemented an algorithm to automatically set an resion of interest on them for Deauville 5-point scaling to ensure objectivity and reproducibility. The two software (SYNAPSE VINCENT and syngo.via) have been tested and verified to display the same value using the same Digital Imaging and COmmunications in Medicine: DICOM storage information. Percentage of BM volume with higher SUL than that of mediastinum (% Higher volume) was calculated, and the Deauville score of Th11 BM for each object was determined according to the previous report [15].

Additionally, some clinical anthropometric and blood-related data including sex, age, LBM, smoking history, WBC count, and hemoglobin level were evaluated.

Statistical analysis

To evaluate the confounding indicators between men and women, the difference between WBC and hemoglobin level (normally distributed) was compared using the t-test, and the differences between other clinical indicators were compared using the Mann–Whitney U test. Multivariate regression analysis was subsequently performed using the clinical indicators that significantly correlated with the PET-derived parameters in the previously performed univariate analyses. Regression analyses between continuous clinical indicators and PET derived parameters were performed using Pearson's correlation coefficient and between smoking habit and PET derived parameters using Spearman's correlation coefficient. In group comparison of the 5-point score and clinical indicators, differences were assessed using a one-way analysis of variance (ANOVA). Chi-square tests, or, for low frequencies, Fisher’s exact test, were used to test for differences between the 5-point scale and smoking habit. One reader performed the entire image processing process twice; the reproducibility of the BM PET extraction was evaluated by the intersection over union for pixel accuracy and the intraclass correlation coefficient for the SULmean. The second procedure was performed at least 30 days following the first analysis, with the order randomly changed. Statistical significance was set at p < 0.05. All analyses were performed using the JMP®15 (SAS Institute Inc., Cary, NC, USA) statistical software.

Results

A total of 98 healthy adults (57 men and 41 women) were enrolled in this study (Table 2). The study participants included 5 (5 women), 18 (12 men, 6 women), 12 (8 men, 4 women), 15 (11 men, 4 women), 25 (14 men, 11 women), 16 (8 men and 8 women), and 7 (3 men and 4 women) participants in their 20 s, 30 s, 40 s, 50 s, 60 s, 70 s, and 80 s, respectively. The average and standard deviation of age was 55.5 ± 14.2 and 56.3 ± 18.9 for men and women, respectively, with no significant difference. The average LBM, and hemoglobin level were significantly different between men and women (p < 0.0001, Table 2). These results indicated that sex was a covariate, and the following univariate and multivariate analyses were performed for each group of men and women.

Table 2 Characteristics of healthy participants

Extraction of BM PET from one subject took about 3 min with the automated process alone, and was completed within 5 min even if manual correction was required. The intersection over union for the extracted pixel accuracy and the intraclass correlation coefficient for the SULmean was conducted for the groups of men and women. The extracted BM PET had a mean intersection over union of 0.99, and a SULmean intraclass correlation coefficient of 0.99, indicating an excellent reproducibility. In 70 of the 98 cases (71.4%), the BM PET was completely extracted automatically. In the other 28 cases (28.6%) that required manual correction (due to high CT values of thyroids: 7, metals for dental treatment: 9, pelvic arterial calcifications: 4, artifacts of pelvic bone: 4, inseparable pelvic and femur: 2, metal for lumbar spine surgery:1, and metal for femur surgery:1), the mean and standard deviation of intersection over union were 0.9875 and 0.0245, respectively. Among the BM PET-derived parameters, the volume, %Higher volume, and Deauville 5-point score were significantly different between men and women (Table 3). The volume increased with increase in LBM in women (r = 0.39, p = 0.0108).

Table 3 Comparison of bone marrow quantitative positron emission tomography (PET) parameters between men and women

Correlation between clinical indicators and PET-derived parameters

The results of the univariate analyses between the clinical indicators and PET parameters are shown in Table 4. Age was associated with SULmean and entropy in both men and women and with SULmax in women. Hemoglobin level was associated with SULmean in men. In a multivariate analysis of SULmean in men, only age was significantly correlated.

Table 4 Univariate analyses between clinical indicators and positron emission tomography (PET)-derived parameters

The measured and predicted values, prediction formulas, and lever ratio plots are shown in Fig. 3 and 4, where r represents the correlation coefficient.

Fig. 3
figure 3

Prediction for mean standardized uptake value corrected by lean body mass (SULmean) (a) and entropy (b) in men

Fig. 4
figure 4

Prediction for maximum standardized uptake value corrected by lean body mass (SULmax) (a), mean standardized uptake value (SULmean) (b) and entropy (c) in women

The representative cases are shown with maximum intensity projection images and the measured PET-derived parameters in Fig. 5. The BM FDG uptake was lower with age and increased with higher LBM.

Fig. 5
figure 5

Maximum intensity projection image and bone marrow positron emission tomography (PET)-derived parameters of two women. FDG uptake in BM of a younger woman (a) was higher both visually and quantitatively (SULmean and % Higher volume) than that of a older woman (b). SUL, standardized uptake value correlated by lean body mass (LBM). White arrows indicate the 11th thoracic vertebral bodies

Discussion

Although the physiological uptake of FDG in the spinal BM has been shown to vary depending on the location of the vertebrae [15], to reduce intra- and interobserver variabilities, the BM extraction of all vertebrae and pelvis was calculated as one organ in this study. Extracted BM images of PET, which almost corresponded to the medullary cavities of the spine and pelvis, were automatically extracted using CT and PET images, and various PET quantitative values were measured. The FDG uptake in BM computed in our study was lower than that in previous reports, even with the difference that our method was corrected for LBM [15, 23] for both sexes. This may be due to background subjective variations and the measurement of larger volumes of BM of areas with low FDG uptake (upper cervical spine, sacrum, and pelvic bone) in the present study, whereas previous reports measured certain bones.

SULmax, SULmean, entropy, and % higher volume of men were higher than those of women, and the proportion of women with a high Deauville score was higher than that of men. These results suggest that although the glucose metabolism of the BM of women is lower than that of men, the bone marrow of women has a relatively high glucose metabolism than other organs. This may be due to the difference in body size between men and women and the presence or absence of menstruation. Since the distribution of normal bone marrow cells is heterogeneous compared to abnormal increases in cell density such as tumors, differences in entropy between men and women may be the same reason. Furthermore, our study showed that FDG uptake in the BM decreased with age in both men and women, it was consistent with the previous studies [21, 23]. Since all of the participants' WBC and hemoglobin levels were in the normal range, the effects of abnormally low and high levels of these hematological indicators on BM uptake could not be fully elucidated. Furthermore, the entropy was also evaluated as one of the histogram features computed from BM PET images. The entropy of BM PET was higher with younger age, indicating heterogeneity in both men and women. Patient-based studies on these findings are warranted.

van Son et al. reviewed previous reports on FDG uptake levels and patterns on FDG PET/CT of hemato-oncological conditions [24]. The review summarized that inflammation and colony-stimulating factors caused a diffuse FDG uptake in the bone marrow that was higher than the physiological FDG uptake in the liver. Diffuse FDG uptake in the BM has also been found in myeloproliferative neoplasms [11, 12], while hyperplastic hematopoietic islands in aplastic anemia showed island-like and localized FDG uptake [13, 25], and BM reconversion with island-like and localized FDG accumulation following the treatment of malignant tumors were observed. Although multiple myeloma had a relatively low diffuse uptake in BM, the CT often showed bone abnormalities such as osteolytic mass and fracture [24]. Bone marrow invasion of malignant lymphoma often presented with localized high uptakes and might also present with diffuse uptakes [24]. Although these findings greatly influence the diagnosis and follow-up of various diseases, quantitative methods have not been established. Therefore, the FDG-uptake pattern in the BM could be characterized by the histogram parameters demonstrated in this study, which represent the FDG uptake’s heterogeneity. The FDG-uptake pattern obtained from this analysis serves as a baseline for future analysis of BM in various hematopoietic disorders.

In this study, we used a commercially available imaging workstation that implemented a recently developed deep learning-based organ segmentation technique for medical imaging [19, 26]. It achieved a highly reproducible and easy extraction of BM PET images of the spine and pelvis from PET/CT images. Although some previously reported computational approaches for bone and BM segmentation from CT-based PET/CT images exist, no direct comparison between these methods and ours was made [16, 17, 27]. Similar to the SUV measurement of malignant solid tumors being widespread in the clinical application of PET, standardization of a simple and accurate quantification method of BM PET for hematopoietic disorders is also necessary.

The major problem of this paper was that there was an insufficient assessment of the mechanism that brings the results of each parameter for each sex. This study evaluated only the bone marrow of healthy adults. The mechanism of various FDG uptake patterns in the bone marrow and its PET parameters can be elucidated by a comparative analysis of abnormal bone marrow accumulation caused by various diseases in the future.

Recent studies have revealed the feasibility of thymidine analog 18F-fluorothymidine (FLT) PET for the diagnosis and monitoring of BM disorders [28, 29]. FLT is a surrogate marker for DNA synthesis and is an excellent PET tracer that can clearly visualize the proliferative activity of the BM; however, it is still not available for other purposes excluding research. Application of the BM quantification method presented in this study to FLT-PET in the future could lead to more useful PET examinations for various hematopoietic disorders.

Our study had some limitations. First, there may have been a selection bias because this was a cross-sectional study using real-world PET imaging data for cancer screening. In addition, the sample size of the survey was small, with less than five participants in their twenties. In addition, some blood data containing C-reactive protein, which is known to correlate with BM-FDG uptake, were not analyzed. Therefore, more participants need to be tested. The blood test did not include CRP or erythrocyte sedimentation rate, which are indicators of inflammation, because it was associated with cancer screening of asymptomatic participants. However, these inflammatory indicators are associated with various diseases and should be included as routine tests. Second, the extracted BM PET contained some bone tissue, and it was not possible to prove that it completely matched the medullary cavity for each individual. Although 2 pixels were removed from the margin on bone CT, the removal of the cortical bone was not perfect because the thickness of the cortical bone could vary by site, age, and sex. However, FDG accumulation in normal bone (rather than bone marrow) is extremely low that it seems that glucose metabolism imaging of bone marrow could be represented almost accurately. In addition, if the area of the spinal canal surrounded by bone was small, it could not be excluded, as shown in Fig. 2a. However, if it was large, it could be excluded, as shown in Fig. 2b. In this study, we did not add manual corrections to establish a simpler method of bone marrow extraction. In the future, it will be improved by applying an algorithm that can accurately extract bone by CT value. Third, the extraction method was not completely automatic and some artifacts were manually removed. This will cause poor reproducibility. CTs reconstructed using the metal artifact reduction algorithm will be useful for fully automated extraction in future studies. Fourth, displacement of PET and CT images due to body movements during PET/CT scans is a major concern. Correction of the PET/CT fusion images using artificial intelligence, which has progressed remarkably, could be possible in the near future.

Conclusions

We presented a simplified FDG-PET/CT BM quantification method and revealed uptake patterns in healthy adults using a histogram analysis. The SULmean and entropy were correlated with age in both men and women, and SULmax was also correlated with age in women. These results could provide baseline findings for various BM abnormalities.

Availability of data and materials

The datasets generated and analyzed during the current study are not publicly available due to the security of data but are available from the corresponding author on reasonable request.

Abbreviations

FDG:

18F-fluorodeoxyglucose

PET:

Positron emission tomography

PET/CT:

Positron emission tomography/computed tomography

SUL:

Standardized uptake value corrected by lean body mass (LBM)

BM:

Bone marrow

WBC:

White blood cell count

FLT:

18F-fluorothymidine

References

  1. Im HJ, Pak K, Cheon GJ, Kang KW, Kim SJ, Kim IJ, et al. Prognostic value of volumetric parameters of (18)F-FDG PET in non-small-cell lung cancer: a meta-analysis. Eur J Nucl Med Mol Imaging. 2015;42:241–51.

    CAS  Article  Google Scholar 

  2. Wen W, Xuan D, Hu Y, Li X, Liu L, Xu D. Prognostic value of maximum standard uptake value, metabolic tumor volume, and total lesion glycolysis of positron emission tomography/computed tomography in patients with breast cancer: a systematic review and meta-analysis. PLoS One. 2019;14:e0225959.

  3. Burggraaff CN, de Jong A, Hoekstra OS, Hoetjes NJ, Nievelstein RAJ, Jansma EP, et al. Predictive value of interim positron emission tomography in diffuse large B-cell lymphoma: a systematic review and meta-analysis. Eur J Nucl Med Mol Imaging. 2019;46:65–79.

    Article  Google Scholar 

  4. Ayubcha C, Hosoya H, Seraj SM, Zadeh MZ, Werner T, Alavi A. The role of (18)F-FDG PET in the assessment of a benign hematological disorder: polycythemia. Hell J Nucl Med. 2019;22:4–5.

    PubMed  Google Scholar 

  5. Ichiki A, Hashimoto N, Ueda T, Hiraiwa S, Tajiri T, Nakamura N, et al. IgG4-related disease with bone marrow involvement. Intern Med. 2016;55:2295–9.

    CAS  Article  Google Scholar 

  6. Inoue K, Goto R, Okada K, Kinomura S, Fukuda H. A bone marrow F-18 FDG uptake exceeding the liver uptake may indicate bone marrow hyperactivity. Ann Nucl Med. 2009;23:643–9.

    Article  Google Scholar 

  7. Yang J, Liu X, Ai D, Fan J, Zheng Y, Li F, et al. PET index of bone glucose metabolism (PIBGM) classification of PET/CT data for fever of unknown origin diagnosis. PLoS One. 2015;10:e0130173.

  8. Pijl JP, Kwee TC, Slart RHJA, Yakar D, Wouthuyzen-Bakker M, Glaudemans AWJM. Clinical implications of increased uptake in bone marrow and spleen on FDG-PET in patients with bacteremia. Eur J Nucl Med Mol Imaging. [in press] 2020;https://doi.org/10.1007/s00259-020-05071-8.

  9. Balogova S, Huchet V, Egrot C, Michaud L, Paycha F, Kerrou K, et al. Effect of erythropoietin on bone marrow uptake of 18F-fluorocholine in prostate cancer: comparison with 18F-fluoride uptake. Clin Nucl Med. 2013;38:200–2.

    Article  Google Scholar 

  10. Okada M, Sato N, Ishii K, Matsumura K, Hosono M, Murakami T. FDG PET/CT versus CT, MR imaging, and 67Ga scintigraphy in the posttherapy evaluation of malignant lymphoma. Radiographics. 2010;30:939–57.

    Article  Google Scholar 

  11. Derlin T, Alchalby H, Bannas P, Veldhoen S, Apostolova I, Triviai I, et al. Assessment of bone marrow inflammation in patients with myelofibrosis: an 18F-fluorodeoxyglucose PET/CT study. Eur J Nucl Med Mol Imaging. 2015;42:696–705.

    CAS  Article  Google Scholar 

  12. Derlin T, Alchalby H, Bannas P, Laqmani A, Ayuk F, Triviai I, et al. Serial 18F-FDG PET for monitoring treatment response after allogeneic stem cell transplantation for myelofibrosis. J Nucl Med. 2016;57:1556–9.

    CAS  Article  Google Scholar 

  13. Chen M, Lu L, Li J, Zhang B, Liu J, Gu J, et al. Value of systemic PET/CT in the diagnosis and differential diagnosis of aplastic anemia. Oncol Lett. 2018;16:3215–22.

    PubMed  PubMed Central  Google Scholar 

  14. Zanoni L, Mattana F, Calabrò D, Paccagnella A, Broccoli A, Nanni C, et al. Overview and recent advances in PET/CT imaging in lymphoma and multiple myeloma. Eur J Radiol. 2021;141:10973.

    Article  Google Scholar 

  15. Shen G, Liang M, Su M, Kuang A. Physiological uptake of 18F-FDG in the vertebral bone marrow in healthy adults on PET/CT imaging. Acta Radiol. 2018;59:1487–93.

    Article  Google Scholar 

  16. Sambuceti G, Brignone M, Marini C, Massollo M, Fiz F, Morbelli S, et al. Estimating the whole bone-marrow asset in humans by a computational approach to integrated PET/CT imaging. Eur J Nucl Med Mol Imaging. 2012;39:1326–38.

    Article  Google Scholar 

  17. Takahashi MES, Mosci C, Souza EM, Brunetto SQ, Etchebehere E, Santos AO, et al. Proposal for a quantitative 18F-FDG PET/CT metabolic parameter to assess the intensity of bone involvement in multiple myeloma. Sci Rep. 2019;9:16429.

    Article  Google Scholar 

  18. Indiran V, Vinod Singh N, Ramachandra Prasad T, Maduraimuthu P. Does coronal oblique length of spleen on CT reflect splenic index? Abdom Radiol (NY). 2017;42(5):1444–8.

    Article  Google Scholar 

  19. Keshwani D, Kitamura Y, Li Y. Computation of total kidney volume from CT images in autosomal dominant polycystic kidney disease using multi-task 3D convolutional neural networks. In: Shi Y, Suk HI, Liu M, editors. Machine learning in medical imaging. Cham: Springer; 2018. p. 380–8.

    Chapter  Google Scholar 

  20. Sharma K, Rupprecht C, Caroli A, Aparicio MC, Remuzzi A, Baust M, et al. Automatic segmentation of kidneys using deep learning for total kidney volume quantification in autosomal dominant polycystic kidney disease. Sci Rep. 2017;7:2049.

    Article  Google Scholar 

  21. Fan C, Hernandez-Pampaloni M, Houseni M, Chamroonrat W, Basu S, Kumar R, et al. Age-related changes in the metabolic activity and distribution of the red marrow as demonstrated by 2-deoxy-2-[F-18]fluoro-D-glucose-positron emission tomography. Mol Imaging Biol. 2007;9:300–7.

    Article  Google Scholar 

  22. van Praagh GD, Nienhuis PH, de Jong DM, Reijrink M, van der Geest KSM, Brouwer E, et al. Toward reliable uptake metrics in large vessel vasculitis studies. Diagnostics (Basel). 2021;11:1986.

    Article  Google Scholar 

  23. Heusch P, Buchbender C, Beiderwellen K, Nensa F, Hartung-Knemeyer V, Lauenstein TC, et al. Standardized uptake values for [18F] FDG in normal organ tissues: comparison of whole-body PET/CT and PET/MRI. Eur J Radiol. 2013;82:870–6.

    Article  Google Scholar 

  24. van Son PM, Evers D, Aarntzen EHJG. The value of FDG-PET CT scans to evaluate bone marrow in haemato-oncological conditions. Neth J Med. 2019;77(7):234–42.

    PubMed  Google Scholar 

  25. Hotta T, Murate T, Inoue C, Kagami T, Tsushita K, Wang JY, et al. Patchy haemopoiesis in long-term remission of idiopathic aplastic anaemia. Eur J Haematol. 1990;45:73–7.

    CAS  Article  Google Scholar 

  26. Masuzawa N, Kitamura Y, Nakamura K, Iizuka S, Simo-Serra E, et al. Automatic segmentation, localization, and identification of vertebrae in 3D CT images using cascaded convolutional neural networks. In: Martel AL, et al., editors. Medical image computing and computer assisted intervention – MICCAI 2020. Cham: Springer; 2020. p. 681–90.

    Chapter  Google Scholar 

  27. Fiz F, Marini C, Campi C, Massone AM, Podestà M, Bottoni G, et al. Allogeneic cell transplant expands bone marrow distribution by colonizing previously abandoned areas: an FDG PET/CT analysis. Blood. 2015;125:4095–102.

    CAS  Article  Google Scholar 

  28. Vercellino L, Ouvrier MJ, Barré E, Cassinat B, de Beco V, Dosquet C, et al. Assessing bone marrow activity in patients with myelofibrosis: results of a pilot study of 18F-FLT PET. J Nucl Med. 2017;58:1603–8.

    CAS  Article  Google Scholar 

  29. Tsujikawa T, Tasaki T, Hosono N, Mori T, Makino A, Kiyono Y, et al. 18F-FLT PET/MRI for bone marrow failure syndrome-initial experience. EJNMMI res. 2019;9:16.

    Article  Google Scholar 

Download references

Acknowledgements

We are thankful to Ms. Kyoko Suzuki and Ken Kodera from FUJIFILM Medical Co. Ltd. for technical support of SYNAPSE VINCENT. We would like to thank Editage (www.editage.jp) for English language editing.

Funding

This research did not receive any specific grant from funding agencies in the public or not-for-profit sectors.

Author information

Authors and Affiliations

Authors

Contributions

YS and HO made substantial contributions to the conception and design of the work, KK acquired data and their analysis, YS and SF analyzed and interpreted the data. YS was major contributors in writing the manuscript. All authors have agreed to submit the final version. In addition, they agreed to take personal responsibility for their contributions and to properly investigate and resolve any questions regarding the accuracy or completeness of any part of the study, even if they were not personally involved, and to document their resolution in the literature. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Yoko Satoh.

Ethics declarations

Ethics approval and consent to participate

This study has been approved by the review board of the Kofu Neurosurgical Hospital to which our institution belongs (approval date: September 16, 2020). Due to the retrospective study design and the use of anonymized patient data, the need for consent to participate was waived by the ethics committee that approved our study. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Satoh, Y., Funayama, S., Onishi, H. et al. Semi-automated histogram analysis of normal bone marrow using 18F-FDG PET/CT: correlation with clinical indicators. BMC Med Imaging 22, 31 (2022). https://doi.org/10.1186/s12880-022-00757-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12880-022-00757-x

Keywords

  • F-18 fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT)
  • Bone marrow
  • Semi-automatic extraction
  • Normal uptake pattern
  • Histogram analysis