Skip to main content

Liver stiffness assessed by real-time two-dimensional shear wave elastography predicts hypersplenism in patients with Wilson’s disease: a prospective study



The current study aimed to explore the value of liver stiffness assessed by two-dimensional real-time shear wave elastography (2D-SWE) to predict hypersplenism occurrence in Wilson’s disease (WD) patients.


Ninety WD patients were enrolled in this prospective study between May 2018 and December 2018. Baseline clinical data and ultrasound imaging including 2D-SWE liver stiffness of WD patients were collected. After enrollment, patients had follow-ups for 24 months or until they developed hypersplenism. The hypersplenism risk factors were determined using Cox regressions and receiver operating characteristic curves (ROC).


Twenty-nine (32.2%) patients developed hypersplenism. Age, portal vein diameter, and liver stiffness were independent hypersplenism risk factors in WD patients. The cutoff value of liver stiffness to predict hypersplenism was 10.45 kPa, with sensitivity and specificity of 75.9% and 73.8%, respectively. Patients were divided into two groups according to liver stiffness: ≥ 10.45 kPa (57.9% with hypersplenism) or < 10.45 kPa (13.5% with hypersplenism). The median time between enrollment and hypersplenism development was 15 months vs. 22 months (p < 0.001) for the two groups, respectively.


The measurement of liver stiffness by 2D-SWE can be a reliable hypersplenism predictor in WD patients. Therefore, dynamic monitoring of WD patients using 2D-SWE is crucial for the early diagnosis of hypersplenism.

Peer Review reports


Wilson’s disease (WD) is a rare autosomal recessive genetic disease [1]. In WD patients, 75% have liver involvement [2], and 34% eventually develop cirrhosis and hypersplenism [3]. WD patients are 4.4 times more likely to develop hypersplenism compared to hepatitis B patients [4]. In liver disease patients, hypersplenism development is determined when they present a leukocyte count < 3500/µL and/or a platelet count < 7.5 × 104/µL [5]. After hypersplenism development, WD patients need be treated with splenectomy [6]. However, patients can have complications such as portal vein thrombosis (PVT) [7] and overwhelming post-splenectomy infection (OPSI) after splenectomy. Also, the mortality rate related to OPSI can be as high as 50% [8]. Therefore, it is of clinical importance to develop techniques to predict whether an individual WD patient will develop hypersplenism. Early intervention with splenic artery embolization can be used to decelerate hypersplenism progression [9].

In previous studies, the assessment of splenic blood flow using four-dimensional (4D) flow magnetic resonance imaging (MRI) has been reported to predict hypersplenism [10]. Patients with high compliance are required in the 4D flow MRI evaluation of splenic blood flow. However, some WD patients could not tolerate MRI examination due to neurological and mental symptoms developed during disease progression [1]. It has been reported that the assessment of liver stiffness by real-time two-dimensional shear wave elastography (2D-SWE) can be applied to predict various hepatic adverse events such as liver cancer caused by hepatitis B and liver failure caused by liver cancer [11, 12], as well as to evaluate whether hepatic patients have complicated portal hypertension [13,14,15]. The Baveno VI criteria (2015) suggests that transient elastography (TE) can clinically identify significant portal hypertension (CSPH) (1b; A) [16]. On this basis, Fofiu R raised 2D-SWE had a good performance to predict high-risk varices (HRV),) including esophageal varices (EV) and gastric varices (GV) in advanced chronic liver disease (cACLD) [17]. However, no attention has yet been paid to the value of 2D-SWE to predict hypersplenism in WD patients mainly due to the rarity of this disease.

Therefore, in the current work, we conducted a prospective longitudinal cohort study to investigate whether liver stiffness assessment by 2D-SWE can be applied to predict hypersplenism in WD patients.



Consecutive WD patients admitted to the Encephalopathy Center of the First Affiliated Hospital, Anhui University of Chinese Medicine, between May 2018 and December 2018 were enrolled. The inclusion criteria consisted of a WD diagnosis with liver involvement according to the Leipzig standard [18] but no hypersplenism at the time of enrollment. Hypersplenism was defined as a leukocyte count < 3500/µL and/or a platelet count < 7.5 × 104/µL [5]. The exclusion criteria were as followed: the presence of other liver diseases (virus hepatitis, alcoholic liver disease, immune liver disease, et al.); severe liver inflammation indicated by ALT elevation greater than five times upper normal limits [19]; failure in liver 2D-SWE assessment; incomplete clinical data at enrollment or during follow-up; did not undergo regular follow up as required; and lost during follow-up. This prospective study was approved by the Institutional Review Board of Ethics Committee of the First Affiliated Hospital, Anhui University of Chinese Medicine (2018AH-08), and informed consent was obtained from all participants.

Clinical and imaging data collection at baseline

In the current study, the starting point for each patient was defined as the day when the patient underwent liver 2D-SWE. At baseline, clinical data including sex, age, body mass index (BMI), presence of Kayser-Fleischer (K-F) ring, fibrosis index based on four factors (FIB-4), and aminotransferase/platelet count ratio index (APRI) were collected. Laboratory results obtained within one week of patient enrollment were considered valid. The portal vein diameter and spleen thickness measured by ultrasound, as well as the liver stiffness assessed by 2D-SWE, were documented.

Assessment of liver stiffness by 2D-SWE

Liver 2D-SWE measurement was performed upon enrollment for each patient. The Mindray-Resona 7 ultrasonic instrument with the SC5-1U probe (Mindray, China) was used and the 2D-SWE mode was adopted. The 2D-SWE measurement was performed by one experienced radiologist (8-year experience of abdominal ultrasound) who had performed more than 300 liver ultrasound examinations in the last two years and at least 50 liver 2D-SWE examinations in the last six months.

Patients were in a fasting state on the morning of the liver 2D-SWE examination. During the 2D-SWE examination, measurements were performed with the WD patient in the supine (76 patients) or slight lateral decubitus position with 30° (14 patients) [19], with the right hand lifted to fully expose the intercostal space. Liver 2D-SWE images were obtained from the right liver lobe through the right intercostal space. The image depth was controlled at 8–10 cm, and the size of the liver 2D-SWE sampling frame was adjusted to 4.0 × 3.0 cm, placed at 1.0 cm below the liver capsule to keep it away from the liver’s great vessels. During 2D-SWE examination, patients were required to hold their breath for 5–6 s in the natural breathing state. The elasticity value was measured when the motion stability index (M-STB) was of five green asterisks and when the reliability map (RLB) changed from purple to green [19]. During measurements, the circular region of interest (ROI) diameter was adjusted to 2.0 cm. Data are displayed in kPa. The average ROI elasticity obtained from each measurement was considered the recorded value of the current measurement (Fig. 1). The measurements were continuously repeated five times, and the median of the recorded values was considered as the patient’s final liver elasticity value. To obtain more accurate and consistent measurement data, we implemented quality control for images and measurements. If the interquartile range (IQR)/median (Med) was higher than 30% after repeated measurements for five consecutive times, the measurement was considered as failed.

Fig. 1
figure 1

Male WD patient, 26, with an average right liver lobe elasticity of 17.16 kPa

Treatment and follow-up strategy

All WD patients received de-coppering therapy during the follow-up period. Patients regularly received dimercaptosuccinic acid (DMSA) orally at a dose of 10 mg/kg/d, bid.

After enrollment, patients were routinely followed up every month. At each visit, leukocyte and platelet counts were assessed to determine whether the patient developed hypersplenism [5]. Patients were prospectively followed up for at least 24 months or until they developed hypersplenism.

Statistical analyses

Measurement data conforming to normal distribution are expressed as means ± SD, and intergroup comparison was performed using a t-test. Measurement data that did not present normal distribution are expressed as medians (IQR), and the Mann–Whitney U test was adopted for inter-group comparison. Enumeration data were expressed as n (%), and inter-group comparison was performed using χ2 or Fisher’s exact tests.

Hypersplenism cumulative incidence was determined based on the hazard ratios derived from Kaplan–Meier survival curves. Cox regression analyses were applied to evaluate the hypersplenism risk factors in WD patients. The receiver operating characteristic curves (ROC) and area under the curves (AUC) were used to calculate the efficacy hypersplenism prediction of liver stiffness in WD patients. Additionally, the threshold, as well as its sensitivity and specificity, were determined by the maximum Youden index. Based on the obtained threshold, a Log-rank test was applied to compare and analyze whether significant differences were present when evaluating the hypersplenism risk between the two groups of WD patients divided by the 2D-SWE threshold. SPSS 25.0 was used for all data analyses. Differences were considered statistically significant when p < 0.05.


General baseline information of the 90 WD patients

Ninety WD patients were eventually included in this study (Fig. 2). Among them, 49 (54.4%) were males and 41 (45.6%) were females, with an average age of 29.8 ± 10.6 years (range: 14–63 years). General baseline information of the 90 patients is summarized in Table 1. The percentage of liver cirrhosis in the hypersplenism group(n = 29) and non-hypersplenism group(n = 61) were 24.1% (7/29) and 19.7% (12/61) respectively.

Fig. 2
figure 2

Flowchart of the study

Table 1 Baseline general information of 90 patients with WD

Reliable liver stiffer assessment by 2D-SWE in WD patients

Five WD patients failed during the liver 2D-SWE examination. They included two patients that did not hold their breaths in the 2D-SWE process, two that presented involuntary movements since neurologic manifestation, and one with 2D-SWE measurements with IQR/M > 30%. We obtained reliable liver stiffer measurements in 90 (94.7%) of 95 patients.

Clinical outcome of the follow-up

During follow-up, 29 (32.2%) patients developed hypersplenism. The median time between the enrollment and the hypersplenism occurrence was 18 months (range, 6–24 months). The cumulative hypersplenism incidence at 6, 12, and 18 months was 4.4%, 21.1%, and 31.1%, respectively (Fig. 3). The remaining 61 (67.8%) patients were continued to follow up without hypersplenism upon a median period of 22 months (range,6–24).

Fig. 3
figure 3

Cumulative hypersplenism incidence in WD patients

Risk factors associated with hypersplenism in WD patients

The Cox regression analyses revealed that age, portal vein diameter, and liver stiffness were independent predictive factors associated with hypersplenism (Table 2). The Cox regression value for the liver stiffness was 0.325, which suggested that with every 1.0 kPa increase in the liver stiffness, the hypersplenism risk increase by 32.5%.

Table 2 Cox regression analysis of risk factors for hypersplenism in WD patients

Value of the liver stiffness measured by 2D-SWE to predict hypersplenism

The ROC analyses reported that the AUCs to predict hypersplenism in the WD patients were 0.433 (95% CI 0.314–0.553) for age, 0.662 (95% CI 0.541–0.782) for spleen vein diameter, and 0.817 (95%CI: 0.727–0.908) for liver stiffness (Fig. 4). Thus, liver stiffness had a superior predictive ability compared to portal vein diameter and age (p < 0.05). The optional liver stiffness cutoff value to predict hypersplenism in WD patients was 10.45 kPa, with sensitivity and specificity of 75.9% and 73.8%, respectively.

Fig. 4
figure 4

Value of age, portal vein diameter, and liver stiffness measured by 2D-SWE to predict hypersplenism in WD patients

To determine the possible association between liver stiffness and the time point of hypersplenism development, the 90 WD patients were divided into high-risk (liver stiffness ≥ 10.45 kPa, n = 38) and low-risk (liver stiffness < 10.45 kPa, n = 52) groups, based on the cutoff value of 10.45 kPa. The cumulative hypersplenism incidence in both groups is shown in Fig. 5. The hypersplenism incidence in the high-risk group was significantly higher compared to the low-risk group (57.9% vs. 13.5%, p < 0.001). The median time between enrollment and the hypersplenism development for the high-risk group was 15 months (range, 6–24 months), and for the low-risk group was 22 months (range, 6–24 months) (p < 0.001) (Fig. 6) (Table 3).

Fig. 5
figure 5

Hypersplenism risk in WD patients in both high- risk (≥ 10.45 kPa) and low-risk (< 10.45 kPa) groups

Fig. 6
figure 6

Baseline 2D-SWE measurement of liver stiffer in two WD patients with different risk of hypersplenism. a The WD patient's right liver lobe elasticity was 9.46 kPa, followed up for 24 months with no hypersplenism occurrence. b The WD patient's right liver lobe elasticity was 11.27 kPa with hypersplenism development at the 13th follow-up month

Table 3 Incidence of hypersplenism in the subgroups with different liver stiffness


In the current study, we explored the value of liver stiffness assessed by 2D-SWE to predict hypersplenism in WD patients. We demonstrated that the assessment of liver stiffness using 2D-SWE is effective for the prospective prediction of hypersplenism in WD patients. Furthermore, WD patients with liver stiffness ≥ 10.45 kPa have a significantly higher hypersplenism risk that might develop in a relatively short term. These findings enabled prompt stratification of WD patients with high or low hypersplenism risk.

We found that age, portal vein diameter, and liver stiffness were independent risk factors for hypersplenism in WD patients. Furthermore, the liver stiffness showed a higher AUC value of 0.817 when compared to age and portal vein diameter. In a previous study, 4D flow MRI was used to predict hypersplenism occurrence in 20 patients. Keller et al. found that the AUC value for splenic artery blood flow was 0.792 [20], slightly lower than that of liver stiffness reported in our current study. MR imaging requires contrast agent injection, has a high cost, and excludes patients with incompatible implanted devices. Besides, WD patients typically develop neurological and psychiatric symptoms during disease progression and might not tolerate MRI examination. Therefore, MRI is a nonideal modality for the assessment of WD patients. Our study suggested that the liver stiffness measured by 2D-SWE can be used to assess the hypersplenism risk in WD patients with high effectiveness and feasibility.

WD is characterized by copper metabolic dysfunction, excessive copper deposition in liver tissues leading to hepatocyte fatty degeneration, chronic inflammation, liver fibrosis, and liver cirrhosis [21]. The WD pathological process is consistent with other chronic liver diseases. The 2D-SWE is a two-dimensional ultrasound-based technology to measure tissue stiffness. It enables real-time analysis of liver stiffness and can uncover the underlying pathological processes of liver diseases. Recently, several studies demonstrated that liver stiffness assessed by 2D-SWE was an alternative tool for physicians to predict disease progression, such as CSPH, EV, and GV [14, 15, 17, 22], avoiding hepatic venous pressure gradient (HPVG) and endoscopy. This is of clinal interest since HPVG and endoscopy are invasive procedures varices [23]. In a previous meta-analysis with 746 liver disease patients, it was suggested that 2D-SWE can be used to forecast portal hypertension with 15.2–24.6 kPa cutoffs, with sensitivities ranging from 78 to 90% and specificities varying from 83 to 89% [20]. Few studies have used 2D-SWE to predict hypersplenism so far. Possibly, 45% of liver diseases (e.g., virus hepatitis) patients complicated with portal hypertension manifested EV and GV but just 7.7% developed hypertension [23]. On the contrary, WD patients complicated with portal hypertension manifested hypersplenism with high incidence of 34% [3]. Our results confirmed that 2D-SWE measurement of liver stiffness in WD patients can effectively predict hypersplenism, extending the application of 2D-SWE in predicting liver diseases complications. We found that the cut-off value of liver stiffness was 10.45 kPa to predict hypersplenism in WD patients, with a sensitivity and specificity of 75.9% and 73.8%, respectively [24]. The discrepancy in cutoff values between our study and the previous meta-analysis can be partially explained by the difference in the average age of patients enrolled in the two studies. The average age of the 746 patients in the aforementioned meta-analysis was 53–71, older than those in our current study (29.8 years).

When compared with patients with liver stiffness < 10.45 kPa, those with ≥ 10.45 kPa had an increased hypersplenism risk (RR 1.385). Besides a relatively higher incidence of hypersplenism (57.9% vs. 13.5%), the median time between baseline and the time points of hypersplenism development in the high-risk patients with liver stiffness ≥ 10.45 kPa was significantly shorter (14 months vs. 20 months, p < 0.001). These results implied that high-risk patients might develop hypersplenism in a relatively short term. Portal hypertension and hypersplenism might be reversible in some WD patients when given glutathione (GSH) intravenously at a dose of 1.8 g in the early stage of hypersplenism [25, 26]. Therefore, the high risk WD patients need more frequent follow-up, allowing for physicians to timely diagnose hypersplenism and adjust treatment options [25, 27]. Our study highlighted that the liver stiffness assessed by 2D-SWE can be applied to monitor liver progression of WD patients by stratifying hypersplenism risk.

Our study has several limitations. Due to the rare occurrence of WD, our results are based on only one medical center. Moreover, we used 2D-SWE data collected by a single experienced radiologist, since guidelines and recommendations state that the intra-observer reproducibility of liver 2D-SWE assessment is excellent [28]. Furthermore, we investigated other laboratory indicators but only FIB-4 and APRI were included for analyses. FIB-4 and APRI are commonly used to monitor liver fibrosis in chronic hepatitis B and C patients as well as WD patients 29 [28]. Whether the 2D-SWE prediction efficacy is improved when more laboratory indicators are included in analyses needs further investigation.


Overall, WD patients with liver stiffness ≥ 10.45 kPa presented a relatively higher risk of hypersplenism. Monitoring liver stiffness with 2D-SWE is essential for WD patients to early diagnose hypersplenism, guide clinical treatment and avoid postoperative complications.

Availability of data and materials

The datasets supporting the conclusions of this article are available from the corresponding author on reasonable request.



Two-dimensional real-time shear wave elastography


Wilson’s disease


Portal vein thrombosis


Overwhelming post-splenectomy infection




Magnetic resonance imaging


Transient Elastography


Clinically significant portal hypertension


High-risk varices


Esophageal varices


Gastric varices


Advanced chronic liver disease


Body mass index

K-F ring:

Kayser-Fleischer ring


Fibrosis index based on four factors


Aminotransferase/platelet count ratio index


Motion stability index


Reliability map


Region of interest


Interquartile range




Dimercaptosuccinic acid


Receiver operating characteristic curve


Area under the curve




  1. Poujois A, Woimant F. Wilson’s disease: a 2017 update. Clin Res Hepatol Gastroenterol. 2018;42(6):512–20.

    Article  CAS  Google Scholar 

  2. Gheorghe L, Popescu I, Iacob S, Gheorghe C, Vaidan R, Constantinescu A, et al. Wilson’s Disease: a challenge of diagnosis. The 5-year experience of a tertiary centre. Rom J Gastroenterol. 2004;13(3):179–85.

    PubMed  Google Scholar 

  3. Roberts EA, Socha P. Wilson disease in children. Handb Clin Neurol. 2017;142:141–56.

    Article  Google Scholar 

  4. Zhong HJ, Sun HH, Xue LF, McGowan EM, Chen Y. Differential hepatic features presenting in Wilson disease-associated cirrhosis and hepatitis B-associated cirrhosis. World J Gastroenterol. 2019;25(3):378–87.

    Article  CAS  Google Scholar 

  5. Ikegami T, Soejima Y, Taketomi A, Kawanaka H, Yoshizumi T, Shimada M, et al. Hypersplenism after living donor liver transplantation. Hepatogastroenterology. 2009;56(91–92):778–82.

    PubMed  Google Scholar 

  6. Li LY, Yang WM, Chen HZ, Wu YH, Fang X, Zhang J, et al. Successful splenectomy for hypersplenism in Wilson’s disease: a single center experience from China. PLoS ONE. 2015;10(4):e014569.

    Google Scholar 

  7. Targarona EM. Portal vein thrombosis after laparoscopic splenectomy: the size of the risk. Surg Innov. 2008;15(4):266–70.

    Article  Google Scholar 

  8. Jones P, Leder K, Woolley I, Cameron P, Cheng A, Spelman D. Postsplenectomy infection—strategies for prevention in general practice. Aust Fam Phys. 2010;39(6):383–6.

    Google Scholar 

  9. Li LY, Chen HZ, Bao YC, Yu QS, Yang WM. Successful treatment of hypersplenism in Wilson’s disease by partial splenic embolization. J Investig Surg. 2018;31(2):75–81.

    Article  Google Scholar 

  10. Collins JD, Ayache Bou JM, Semaan E, Salem R, Carr JC, Markl M, et al. Quantitative assessment of splenic hemodynamics at 4D flow MRI to diagnose hypersplenism associated thrombocytopenia. J Vasc Interv Radiol. 2015;26:S105–6.

    Article  Google Scholar 

  11. Shen Y, Zhou C, Zhu G, Shi G, Zhu X, Huang C, et al. Liver stiffness assessed by shear wave elastography predicts postoperative liver failure in patients with hepatocellular carcinoma. J Gastrointest Surg. 2017;21(9):1471–9.

    Article  Google Scholar 

  12. Jeong JY, Sohn JH, Sohn W, Park CH, Kim TY, Jun DW, et al. Role of shear wave elastography in evaluating the risk of hepatocellular carcinoma in patients with chronic hepatitis B. Gut Liver. 2017;11(6):852–9.

    Article  CAS  Google Scholar 

  13. Yoon HM, Kim SY, Kim KM, Oh SH, Ko GY, Park Y, et al. Liver stiffness measured by shear-wave elastography for evaluating intrahepatic portal hypertension in children. J Pediatr Gastroenterol Nutr. 2017;64(6):892–7.

    Article  Google Scholar 

  14. Procopet B, Berzigotti A, Abraldes JG, Turon F, Hernandez-Gea V, Garcia-Pagan JC, et al. Real-time shear-wave elastography: applicability, reliability and accuracy for clinically significant portal hypertension. J Hepatol. 2015;62(5):1068–75.

    Article  Google Scholar 

  15. Grgurevic I, Bokun T, Mustapic S, Trkulja V, Heinzl R, Banic M, et al. Real-time two-dimensional shear wave ultrasound elastography of the liver is a reliable predictor of clinical outcomes and the presence of esophageal varices in patients with compensated liver cirrhosis. Croat Med J. 2015;56(5):470–81.

    Article  CAS  Google Scholar 

  16. de Franchis R, Baveno VIF. Expanding consensus in portal hypertension: report of the Baveno VI Consensus Workshop: stratifying risk and individualizing care for portal hypertension. J Hepatol. 2015;63(3):743–52.

    Article  Google Scholar 

  17. Fofiu R, Bende F, Popescu A, Sirli R, Miutescu B, Sporea I. Assessing Baveno VI criteria using liver stiffness measured with a 2D-shear wave elastography technique. Diagnostics (Basel). 2021;11(5):737.

    Article  Google Scholar 

  18. Ferenci P, Caca K, Loudianos G, Mieli-Vergani G, Tanner S, Sternlieb I, et al. Diagnosis and phenotypic classification of Wilson disease. Liver Int. 2003;23(3):139–42.

    Article  Google Scholar 

  19. Barr RG, Wilson SR, Rubens D, Garcia-Tsao G, Ferraioli G. Update to the society of radiologists in ultrasound liver elastography consensus statement. Radiology. 2020;296(2):263–74.

    Article  Google Scholar 

  20. Keller EJ, Kulik L, Stankovic Z, Lewandowski RJ, Salem R, Carr JC, et al. JOURNAL CLUB: four-dimensional flow MRI-based splenic flow index for predicting cirrhosis-associated hypersplenism. AJR Am J Roentgenol. 2017;209(1):46–54.

    Article  Google Scholar 

  21. Ferenci P. Pathophysiology and clinical features of Wilson disease. Metab Brain Dis. 2004;19(3–4):229–39.

    Article  CAS  Google Scholar 

  22. Maruyama H, Kobayashi K, Kiyono S, Sekimoto T, Kanda T, Yokosuka O. Two-dimensional shear wave elastography with propagation-based reliability assessment for grading hepatic fibrosis and portal hypertension. J Hepatobiliary Pancreat Sci. 2016;23(9):595–602.

    Article  Google Scholar 

  23. Fofiu R, Bende F, Popescu A, Sirli R, Lupusoru R, Ghiuchici AM, et al. Spleen and liver stiffness for predicting high-risk varices in patients with compensated liver cirrhosis. Ultrasound Med Biol. 2021;47(1):76–83.

    Article  Google Scholar 

  24. Suh CH, Kim KW, Park SH, Lee SS, Kim HS, Tirumani SH, et al. Shear wave elastography as a quantitative biomarker of clinically significant portal hypertension: a systematic review and meta-analysis. Am J Roentgenol. 2018;210(5):W185–95.

    Article  Google Scholar 

  25. Aggarwal A, Bhatt M. Advances in treatment of Wilson disease. Tremor Other Hyperkinet Mov (N Y). 2018;8:525.

    Article  Google Scholar 

  26. Dietrich CF, Bamber J, Berzigotti A, Bota S, Cantisani V, Castera L, et al. EFSUMB guidelines and recommendations on the clinical use of liver ultrasound elastography, update 2017 (long version). Ultraschall Med. 2017;38(4):e48.

    Article  Google Scholar 

  27. Kozic DB, Semnic R, Petrovic I, Svetel M, Ostojic J, Kostic VS. Are irreversible morphological [corrected] signs of portal hypertension in neurological form of Wilson’s disease associated with treatment delay? A pilot study. Acta Neurol Belg. 2012;112(3):261–4.

    Article  CAS  Google Scholar 

  28. Hwang J, Yoon HM, Jung AY, Lee JS, Kim KM, Oh SH, et al. Diagnostic performance of ultrasound elastography and serologic fibrosis indices for evaluation of hepatic involvement in Wilson disease. J Ultrasound Med. 2020;39(11):2231–42.

    Article  Google Scholar 

Download references


The authors appreciate the technical support of Dr. Wenshuo Tian and Dr. Cuimei Lou (Mindray medical).


University Natural Science Research Project of Anhui Province, China (KJ2020A0436). The funding body played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

Author information

Authors and Affiliations



All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by JW, MH, QZ and LS. The first draft of the manuscript was written by JW and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Qiang Zhu.

Ethics declarations

Ethics approval and consent to participate

In this study, all procedures abided by the protocol of the First Affiliated Hospital, Anhui University of Chinese Medicine. The institutional review board approved this study. Ethical approval was approved by the Ethical Committee of the hospital, and obtained ethics approval in written (2018AH-08). Written informed consent to participate was obtained from all the patients for publication of this study.

Consent for publication

Participants of this study have provided written consent for publication.

Competing interests

The authors declare that there are no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Wang, J., Hu, M., Zhu, Q. et al. Liver stiffness assessed by real-time two-dimensional shear wave elastography predicts hypersplenism in patients with Wilson’s disease: a prospective study. BMC Med Imaging 22, 25 (2022).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • Wilson’s disease
  • Hypersplenism
  • Liver stiffness
  • Shear wave elastography