- Research article
- Open Access
Reproducibility of small animal cine and scar cardiac magnetic resonance imaging using a clinical 3.0 tesla system
BMC Medical Imaging volume 13, Article number: 44 (2013)
To evaluate the inter-study, inter-reader and intra-reader reproducibility of cardiac cine and scar imaging in rats using a clinical 3.0 Tesla magnetic resonance (MR) system.
Thirty-three adult rats (Sprague–Dawley) were imaged 24 hours after surgical occlusion of the left anterior descending coronary artery using a 3.0 Tesla clinical MR scanner (Philips Healthcare, Best, The Netherlands) equipped with a dedicated 70 mm solenoid receive-only coil. Left-ventricular (LV) volumes, mass, ejection fraction and amount of myocardial scar tissue were measured. Intra-and inter-observer reproducibility was assessed in all animals. In addition, repeat MR exams were performed in 6 randomly chosen rats within 24 hours to assess inter-study reproducibility.
The MR imaging protocol was successfully completed in 32 (97%) animals. Bland-Altman analysis demonstrated high intra-reader reproducibility (mean bias%: LV end-diastolic volume (LVEDV), -1.7%; LV end-systolic volume (LVESV), -2.2%; LV ejection fraction (LVEF), 1.0%; LV mass, -2.7%; and scar mass, -1.2%) and high inter-reader reproducibility (mean bias%: LVEDV, 3.3%; LVESV, 6.2%; LVEF, -4.8%; LV mass, -1.9%; and scar mass, -1.8%). In addition, a high inter-study reproducibility was found (mean bias%: LVEDV, 0.1%; LVESV, -1.8%; LVEF, 1.0%; LV mass, -4.6%; and scar mass, -6.2%).
Cardiac MR imaging of rats yielded highly reproducible measurements of cardiac volumes/function and myocardial infarct size on a clinical 3.0 Tesla MR scanner system. Consequently, more widely available high field clinical MR scanners can be employed for small animal imaging of the heart e.g. when aiming at serial assessments during therapeutic intervention studies.
The accurate and reproducible assessment of cardiac function by measurement of leftventricular (LV) volumes, ejection fraction, and the amount of myocardial scar tissue is fundamental for evaluating heart diseases in cardiovascular research. A great advantage of cardiac magnetic resonance (CMR) imaging is the inherently high soft tissue contrast, and the possibility to image cardiac and vascular anatomy in a three-dimensional manner. The accuracy of CMR imaging for the evaluation of LV volumes, mass and scar amount has been validated in several human and small animal studies [1–6]. However, most animal studies have been conducted on dedicated ultra-high field small animal MR systems which are not widely available [6–8]. Thus, cardiac imaging in rodents using conventional clinical MR scanners would be highly desirable but remains technically demanding since imaging at high heart rates (> 300/min) will inadvertently limit the required high spatial and temporal resolution . Recently, clinical MR scanners have been successfully employed to overcome these difficulties [10–12]. Cardiovascular research generally relies on reliable and accurate imaging protocols and such imaging protocols are mandatory for small animal examinations either; in particular the exact determination of LV volumes/mass and myocardial infarct size plays a pivotal role in therapeutic intervention studies . In order to facilitate accurate and reproducible cardiac small animal imaging on commonly available clinical MR scanners, the present study determined inter-study, inter-reader and intra-reader reproducibility for the evaluation of LV volumes, mass and myocardial scar size in rats using a clinical 3.0 Tesla MR system.
The study was approved by the Charité Institutional Review Board. Thirty-three adult Sprague–Dawley rats (7–16 weeks old) with a mean weight of 355.5 ± 91.8 g (range 269–589 g) were included in our study protocol. For surgical intervention, the animals were anesthetized with 0.3 ml Domitor® (1 mg/1 mL) + 0.8 ml Dormicum® (5 mg/1 mL)/kg BW. After thoracotomy, the left anterior descending coronary artery (LAD) was occluded on the level of the first diagonal branch by placing a snare ligature around the artery and a small portion of the surrounding myocardium. All animals underwent CMR imaging within 24 hours after surgery.
CMR imaging was performed using a clinical 3.0 Tesla MR system (Philips Healthcare, Best, The Netherlands) equipped with a Quasar Dual gradient system (40 mT/m, slew rate 200 T/m/sec) using a dedicated 70 mm solenoid receive-only coil placed perpendicular to B0 providing a high signal-to-noise ratio (Philips Research Laboratory, Hamburg, Germany). Anaesthesia was induced and maintained during the CMR examination with 3% isoflurane administered by a nasal cone. The animals were placed in a specially-designed cradle with four neonatal ECG electrodes (3 M™ Red Dot™ 2269 T) being attached to the paws in order to record an electrocardiogram for triggering of image data acquisition (see Figure 1). A dedicated software patch (GyroTools Ltd., Zurich Switzerland) installed on the scanner console enabled triggered image data acquisition up to heart rates of 400 beats per minute which constituted the only modification applied to the commercially available standard software of the scanner system. A multi-stack, multi-slice, segmented, spoiled gradient echo (GRE) pulse sequence was performed for localization of the heart in the three standard planes (transverse, sagittal, and coronal). For the assessment of LV volumes/mass whole heart coverage with contiguous short-axis cine imaging (8–10 slices) was carried out followed by acquisition of standard 4-and 2-chamber views.
For cine imaging a segmented gradient echo sequence was applied (turbo field echo; repetition time/echo time, 9.0 ms/4.2 ms; flip angle, 15°; temporal resolution 9.9 ms; in-plane spatial resolution, 0.4 x 0.4 mm2; slice thickness, 1.5 mm; field-of-view, 64 mm2; matrix, 159; total scan time approximately 20 minutes). Following cine imaging, a bolus of gadolinium-DTPA (dosage, 0.5 mmol/100 g BW) was administered via a catheter placed in a tail vein. After a 15 minute waiting period, full left-ventricular coverage, three-dimensional late enhancement (LE) imaging was performed in identical short axis geometry using an inversion prepared segmented gradient-echo-sequence (data acquisition window, 32 ms; time between 180° pulses = 2 heart beats; echo time, 3.3 ms; flip angle, 15°; in-plane spatial resolution, 0.3 x 0.3 mm2; slice thickness, 1.6 mm; field-of-view, 55 mm2; matrix, 188; total scan time approximately 14 minutes). The delay of the inversion prepulse was determined from an inversion prepared cine scan (Look-Locker sequence) and individually adjusted to optimally suppress signal from normal myocardium. All acquisitions were performed during free breathing with respiratory motion being partially compensated for by employing signal averaging (Number of Signal Averages, NSA = 5 for cine imaging and NSA = 2 for LE imaging).
All data sets were evaluated using a standard analysis software (ViewForum Release 5.1, Philips Healthcare, Best, The Netherlands).
Assessment of LV volumes
Endocardial borders were traced manually at end-diastole and end-systole (Figure 2) with endocardial trabeculae being excluded; basal short axis slices demonstrating > 50% of visible circumferential LV myocardium were included in the analysis. LV end-diastolic volume (LVEDV) and LV end-systolic volume (LVESV) were calculated by applying Simpson’s rule with LV ejection fraction (LVEF) derived accordingly. In addition, end-diastolic epicardial borders were traced manually for measurement of end-diastolic LV mass (specific myocardial density, 1.05 g/cm3). LV end-diastolic diameter (LVEDD) was measured in a basal short-axis view according to standard definitions.
Assessment of LV scar amount
Endo-and epicardial borders were manually traced on LE images. The amount of scar was quantified by manual segmentation with the signal intensity threshold set to >2 standard deviations above the mean signal intensity of a remote myocardial region (Figure 3). The volume of enhanced myocardium was determined according to the disc summation method and provided in microliter (μl); in addition percentage of scar of total LV mass was calculated.
The intra-reader and inter-reader reproducibility of LV volumes and mass were evaluated in all animals. To assess intra-reader reproducibility, left-ventricular contours were redrawn by the original observer (T.H., >4 years experience in reading >500 CMR studies per year) one month after the initial analysis. In order to assess inter-reader reproducibility an additional observer (R.M., >7 years experience in reading >600 CMR studies per year) carried out a separate analysis. For the assessment of inter-study reproducibility, 6 randomly chosen animals were scanned twice within 24 hours and evaluated by the first observer being blinded to the animal data.
Statistical analyses were performed using SPSS, version 17.0, software (SPSS, Chicago). Values are reported as means ± standard deviations. The mean bias between the reads and their limits of agreement were determined according to the method described by Bland and Altman  and by calculating Lin's concordance coefficient  using MedCalc® Version 18.104.22.168 (MedCalc Software, Belgium). In addition, the coefficient of variability (CV) was derived . All tests were two sided, and P < 0.05 was considered to indicate statistical significance.
Image data acquisition was successfully completed in 32 out of 33 animals (97%) and in all 6 animals (100%) undergoing repeat CMR examinations. Heart rate ranged from 270 to 310 beats per minute (bpm), one CMR examination suffered from ECG-related triggering problems and could not be finalized. All 32 completed CMR examinations were of diagnostic image quality and constituted the final study population for analysis (for a representative imaging example see Figure 4 and Additional file 1).
Left ventricular measurements
LVEF, LVEDV and LVEDD ranged from 23.0 to 68.8%, 257.0 to 609.2 μl and 5.3 to 8.7 mm, respectively; LV mass demonstrated values between 199.4 and 450.4 mg and myocardial scar volume ranged from 0 to 99.9 μl. The absolute values of all parameters are summarized in Table 1.
Intra- and inter-reader reproducibility
Bland-Altman plots of intra-and inter-reader agreement are shown in Figure 5A and B, respectively. Calculation of Lin’s concordance correlation coefficient confirmed a substantial intra-reader agreement with regard to LV volume assessment and myocardial scar amount (range, 0.92 to 0.97); inter-reader agreement for the assessment of LV volumes and scar amount was moderate (range, 0.82 to 0.95, see Table 1).
The bias of repeat CMR examinations was found to be minimal for all left-ventricular parameters (Table 2). In addition, calculation of Lin’s concordance correlation coefficient resulted in a substantial inter-study agreement for the assessment of LV volumes and scar amount (range, 0.84 to 0.96, see Table 2).
The present study validated the use of a clinical 3.0 Tesla MR system for cardiac cine and scar imaging in small animals. Primarily, small rodent imaging is performed on dedicated animal MR systems with field strengths ≥ 4.7 Tesla with gradient and coil systems especially developed for small animal imaging. However, these MR scanner systems are not widely available and beyond that, require expensive investments. To overcome these restrictions, clinical MR systems may have the potential to represent a valuable alternative for accurate assessment of cardiovascular anatomy and function in small rodents. However, several key items need to be considered when performing CMR examinations in small animals: First, robust ECG monitoring is essential to ensure reliably triggered image data acquisition. Second, anaesthesia and animal temperature have to be monitored and maintained throughout the complete CMR examination in particular when aiming at serial examinations. Finally, in order to achieve an optimal signal-to-noise ratio resulting in high quality CMR images, high magnetic field strengths need to be combined with dedicated small animal radiofrequency receiver coils. In the present study, a clinically employed 3.0 Tesla system was introduced for the purpose of small animal imaging. In order to increase image SNR and minimize breathing artefacts, signal averaging was mandatory thereby accepting an increased total scan duration. In addition, for cine imaging a segmented gradient echo sequence was chosen over the commonly used steady-state free precession (SSFP) sequence type since the latter resulted in poor image quality mainly due to flow-/bloodpool-related artifacts with impairment of endocardial visibility (e.g. in the region of mitral inflow/aortic root). However, small animal CMR imaging at 3.0 Tesla proved to be feasible with a success rate of >97%. Recently, Saleh et al.  proved that long-term left ventricular remodelling in rat hearts after myocardial infarction can be rapidly and accurately assessed using a clinical 3.0 Tesla System with a dedicated small animal coil and a gradient echo sequence. In addition, quantitative assessment of cardiac volumes, mass and infarct size provided robust results with high inter-study, inter-reader and intra-reader reproducibility. The high reproducibility for the quantification of left-ventricular volumes and mass was in accordance to previously published data by Montet-Abou et al.  who investigated nine normal rats with four standard MR fast gradient- echo sequences using a 1.5 Tesla MR system. However, inter-, and intra-reader reproducibility was limited to four rats. Jones et al.  investigated 11 rats in an ischemia-reperfusion model and showed similar inter-study (3.3% vs. 4.8%) and intra-study (1.6% vs. 1.0%) reproducibility for the assessment of LVEF.
Accurate depiction of myocardial infarct size in animal models of cardiac diseases is crucial; hence, late enhancement imaging was incorporated in the current study protocol. Recently, Voelkl et al.  showed that widely available clinical 1.5 Tesla scanners enable high quality and accurate assessment of infarct size and functional parameters in mice. In our study quantitative analysis of myocardial infarct size demonstrated high inter-study, inter-reader and intra-reader reproducibility. The overall high reproducibility of quantitative CMR measurements may allow for the reliable and accurate assessment of even small treatment effects during serial examinations with the additional benefit of reducing sample size. In general, CMR imaging has been proposed the imaging method of choice to assess treatment effects in clinical and animal studies due to its high accuracy and low inter-study variability [6, 16]. Based on the results of the present study conducted with the use of a conventional clinical 3.0 Tesla MR system a more widespread use of CMR imaging in small animal studies can be encouraged. In particular, the combined protocol of functional and morphological imaging of the rat heart is advantageous and can be integrated in pharmacological or interventional treatment studies in small animals.
Even though clinical MR scanners are more abundant than dedicated animal MR scanners, they may not always be available for animal studies and/or local rules may prevent their use. Image quality of small animal imaging on clinical scanners is generally restricted by limited shim capabilities which may be addressed by introducing high-order shimming. In addition, for small objects like rats and mice, the noise not only arises from physiological and thermal object noise but is rather dominated by noise from the electronic pathways; hence, a fully digital broadband system with analog to digital signal conversion in the radiofrequency coil may prove beneficial. Taking advantage of such technical improvements may allow for example to use steady-state free precession imaging sequences.
The present study showed that a conventional clinical 3.0 Tesla MR scanner environment can be adapted to achieve high quality functional and morphological cardiac MR imaging of rats in vivo. Measurements of LV functional parameters and scar amount were highly reproducible. Consequently, the performance of therapeutic interventions aiming at the improvement of contractile function and the reduction of myocardial scar amount (e.g. stem cell therapy) may be studied in small animal models using widely available clinical CMR scanners.
Daire JL, Jacob JP, Hyacinthe JN, Croisille P, Montet-Abou K, Richter S, Botsikas D, Lepetit-Coiffe M, Morel D, Vallee JP: Cine and tagged cardiovascular magnetic resonance imaging in normal rat at 1.5 T: a rest and stress study. J Cardiovasc Magn Reson. 2008, 10: 48-10.1186/1532-429X-10-48.
Fieno DS, Jaffe WC, Simonetti OP, Judd RM, Finn JP: TrueFISP: assessment of accuracy for measurement of left ventricular mass in an animal model. J Magn Reson Imaging. 2002, 15: 526-531. 10.1002/jmri.10107.
Goetti R, Kozerke S, Donati OF, Surder D, Stolzmann P, Kaufmann PA, Luscher TF, Corti R, Manka R: Acute, subacute, and chronic myocardial infarction: quantitative comparison of 2D and 3D late gadolinium enhancement MR imaging. Radiology. 2011, 259: 704-711. 10.1148/radiol.11102216.
Kim RJ, Chen EL, Lima JA, Judd RM: Myocardial Gd-DTPA kinetics determine MRI contrast enhancement and reflect the extent and severity of myocardial injury after acute reperfused infarction. Circulation. 1996, 94: 3318-3326. 10.1161/01.CIR.94.12.3318.
Rehr RB, Malloy CR, Filipchuk NG, Peshock RM: Left ventricular volumes measured by MR imaging. Radiology. 1985, 156: 717-719.
Yang Z, Berr SS, Gilson WD, Toufektsian MC, French BA: Simultaneous evaluation of infarct size and cardiac function in intact mice by contrast-enhanced cardiac magnetic resonance imaging reveals contractile dysfunction in noninfarcted regions early after myocardial infarction. Circulation. 2004, 109: 1161-1167. 10.1161/01.CIR.0000118495.88442.32.
Krombach GA, Saeed M, Higgins CB, Novikov V, Wendland MF: Contrast-enhanced MR delineation of stunned myocardium with administration of MnCl(2) in rats. Radiology. 2004, 230: 183-190. 10.1148/radiol.2301020228.
Dietrich T, Hucko T, Bourayou R, Jahnke C, Paetsch I, Atrott K, Stawowy P, Grafe M, Klein C, Schnackenburg B, et al: High resolution magnetic resonance imaging in atherosclerotic mice treated with ezetimibe. Int J Cardiovasc Imaging. 2009, 25: 827-836. 10.1007/s10554-009-9487-5.
Vallee JP, Ivancevic MK, Nguyen D, Morel DR, Jaconi M: Current status of cardiac MRI in small animals. MAGMA. 2004, 17: 149-156. 10.1007/s10334-004-0066-4.
Saleh MG, Sharp SK, Alhamud A, Spottiswoode BS, van der Kouwe AJ, Davies NH, Franz T, Meintjes EM: Long-term left ventricular remodelling in rat model of nonreperfused myocardial infarction: sequential MR imaging using a 3 T clinical scanner. J Biomed Biotechnol. 2012, 2012: 504037-
Campan M, Lionetti V, Aquaro GD, Forini F, Matteucci M, Vannucci L, Chiuppesi F, Di Cristofano C, Faggioni M, Maioli M, et al: Ferritin as a reporter gene for in vivo tracking of stem cells by 1.5-T cardiac MRI in a rat model of myocardial infarction. Am J Physiol Heart Circ Physiol. 2011, 300: H2238-2250. 10.1152/ajpheart.00935.2010.
Voelkl JG, Haubner BJ, Kremser C, Mayr A, Klug G, Loizides A, Muller S, Pachinger O, Schocke M, Metzler B: Cardiac imaging using clinical 1.5 t MRI scanners in a murine ischemia/reperfusion model. J Biomed Biotechnol. 2011, 2011: 185683-
Gilson WD, Kraitchman DL: Cardiac magnetic resonance imaging in small rodents using clinical 1.5 T and 3.0 T scanners. Methods. 2007, 43: 35-45. 10.1016/j.ymeth.2007.03.012.
Bland JM, Altman DG: Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986, 1: 307-310.
Lin LI: A concordance correlation coefficient to evaluate reproducibility. Biometrics. 1989, 45: 255-268. 10.2307/2532051.
Grothues F, Smith GC, Moon JC, Bellenger NG, Collins P, Klein HU, Pennell DJ: Comparison of interstudy reproducibility of cardiovascular magnetic resonance with two-dimensional echocardiography in normal subjects and in patients with heart failure or left ventricular hypertrophy. Am J Cardiol. 2002, 90: 29-34. 10.1016/S0002-9149(02)02381-0.
Montet-Abou K, Daire JL, Ivancevic MK, Hyacinthe JN, Nguyen D, Jorge-Costa M, Morel DR, Vallee JP: Optimization of cardiac cine in the rat on a clinical 1.5-T MR system. MAGMA. 2006, 19: 144-151. 10.1007/s10334-006-0037-z.
Jones JR, Mata JF, Yang Z, French BA, Oshinski JN: Left ventricular remodeling subsequent to reperfused myocardial infarction: evaluation of a rat model using cardiac magnetic resonance imaging. J Cardiovasc Magn Reson. 2002, 4: 317-326. 10.1081/JCMR-120013297.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2342/13/44/prepub
The authors declare that they have no competing interests.
RM, CJ, IP, BS and KG conceived of the study, participated in its design and coordination and performed the statistical analysis. RM, CJ, RG and IP carried out the imaging studies and drafted the manuscript. RM and TH carried out image analysis. TH and TD carried out animal preparation and experimental setup. All authors read and approved the final manuscript.
Robert Manka, Cosima Jahnke contributed equally to this work.
Authors’ original submitted files for images
Additional files 1: Comparison of cine and late enhancement imaging. Cine and late enhancement imaging revealed akinetic wall motion and corresponding myocardial scar tissue resulting from myocardial infarction (same animal as in Figure 4). (MP4 165 KB)
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Manka, R., Jahnke, C., Hucko, T. et al. Reproducibility of small animal cine and scar cardiac magnetic resonance imaging using a clinical 3.0 tesla system. BMC Med Imaging 13, 44 (2013). https://doi.org/10.1186/1471-2342-13-44
- Cardiac Magnetic Resonance
- Myocardial Infarct Size
- Magnetic Resonance System
- Small Animal Imaging
- Cardiac Magnetic Resonance Study