Fig. 1

1 remedial case achieved by CEUS-guided puncture. The case was a patient who had a space-occupying lesion in the upper pole of the right kidney, about 9.5 × 7.8 cm in size, with irregular shape and unclear boundary. The liquefaction area was not found by grayscale ultrasound. (a) The right kidney biopsy guided by conventional ultrasound was performed for the first time. (b) The effective tissue components of the first puncture specimen were few and no tumor components were found under the pathological microscope (HE staining, ×200 times). (c-d) CEUS in the second biopsy showed large necrosis in the lesion, and the puncture target was located in the area with contrast agent perfusion. (e) The puncture biopsy of the enhanced area in the lesion was carried out immediately. (f) Under the pathological microscope, diffuse infiltration of tumor cells stained with cytoplasm (HE staining, ×200 times) could be seen