Fig. 5

Hematoxylin and eosin staining images (× 200) of the left kidneys in the C, I, D, and DI groups at week 0, 4, 8, and 12. The morphology and structure of glomeruli and renal tubules were normal in the C group from week 0 to 12 and the I, D, and DI groups at week 0. There was edema and ballooning degeneration of few renal tubular epithelial cells in the I, D, and DI groups at week 4. The edema of tubular epithelial cells was more obvious and extensive in the I, D, and DI groups at week 8. There was necrosis of few tubular epithelial cells, the infiltration of inflammatory cells in interstitial substance, glomerular shrinkage, and interstitial fibrosis in the I, D, and DI groups at week 12. The degree of kidney injury and fibrosis was more severe in the DI group than those in the I and D groups