Fig. 6

Nucleus segmentation steps. From the left, First row: Original images of eosinophils (first and second columns), lymphocyte (third column), monocyte (fourth column), and neutrophils (fifth column). Second row: Histogram decomposed and approximation. The dotted vertical lines indicated the values of \(T_{nc0}\) and the local minimum respectively. The un-dotted vertical line indicates the estimated threshold \({\tilde{\varepsilon }}_{t}\). Third row: Binarized image using estimated threshold \({\tilde{\varepsilon }}_{t}\). Fourth row: Segmented nucleus after post-processing. The results were obtained by setting \(Er= 0.07\)