Quantification of liver iron overload disease with laser ablation inductively coupled plasma mass spectrometry

BMC Medical Imaging

Table 1 Patient's characteristics

Cohort	Diagnosis	HFE Genetics*	Sex	Age	Liver tissue Fe [μg/g]	Serum Fe [μM]	Transferrin [mg/dL]	Ferritin [μg/L]	Tranferrin saturation [%]	Anonymised Sample no.
Iron overload	HFE HH	Y282/Y282 H63/H63	M	61	1117	ND	138	1950	95	H4
	Non-HFE HH	C282/C282 H63/D63	M	55	2828	22.4	246	1850	36	H3
	HFE HH	Y282/Y282 H63/H63	F	60	7600	33.7	158	934	85	H1
	HCV	C282/C282 H63/H63	F	13	NID	21.4	246	52	35	H2
Normal iron status	HBV, LTx	ND	M	59	NID	16.9	254	52	26	C4
	Ductopenia, LTx	ND	F	69	NID	17.4	190	196	36	C1
	LTx	ND	F	53	NID	21.0	297	64	28	C3
	GvH	ND	M	36	NID	19.2	250	1431	31	C2

Abbreviations used: F female, GvH graft-versus-host, HBV hepatitis B virus, HCV hepatitis C virus, HFE HH hereditary hemochromatosis, LTx liver transplantation, M male, NA not applicable, ND not determined, NID histologically no iron deposition, NN not known. Normal laboratory reference ranges are: hepatic Fe [μg/g dry weight]: 200-2400 (M), 400-1600 (F); serum Fe [μM]: 9-29 (M), 9-27 (F); Transferrin [mg/dL]: 200-400; Ferritin [μg/L]: 30-300 (M), 10-200 (F); Transferrin saturation [%]: 18-45. * HFE genotype constellations of patients subjected to LA-ICP-MS analysis were determined using TaqMan allelic discrimination assay [3, 9]

ISSN: 1471-2342