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Figure 5 | BMC Medical Imaging

Figure 5

From: Computer-assisted assessment of the Human Epidermal Growth Factor Receptor 2 immunohistochemical assay in imaged histologic sections using a membrane isolation algorithm and quantitative analysis of positive controls

Figure 5

Variations on the Membrane Staining Intensities of Positive Control Specimens. This is a histogram showing the amount of specimens in each range of intensities. Each bar contains cases that lie within a 20 unit range of intensities. Though most positive controls stain darkly (215–235), there is a significant number of cases in which staining intensity deviates markedly from the mode. Consequently, features that take into account the intensity of the positive control are used in this study.

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