Figure 3

Histology image segmentation into chromatin-rich, stromal, and unstained regions using image intensity. A grayscale value is computed for each pixel as the average of red, green, and blue color channels, and a 3 cluster k-means algorithm is used to determine the intensity thresholds between the respective clusters. The lowest intensity range is associated with the cell populated regions, while the highest intensity range determines the adipose tissue and the background. The middle intensity range corresponds to the stroma.