Histology with Hematoxilin and Eosin (H&E) staining. A low power objective (A, 10X) showed areas of granulomatous inflammation. At a higher magnification (B, H&E stain, 40X) (C, H&E stain, 60X), several spherules (arrows) could be clearly identified. Spherule (arrow) and endospores (dot arrow) are also visible by the Gomori’s methenamine silver (GMS) stain (D, 40X). The size and morphology of these structures is consistent with Coccidioides immitis.