Quantitative metric profiles capture three-dimensional temporospatial architecture to discriminate cellular functional states

Table 1 Cell Culture Conditions

Name	Cell Type	Media
MCF10A	Precancerous Human	Dulbecco's Minimum Essential Media (DMEM)/F12,
	Breast Epithelial	5%Horse Serum (HS), 1% Penicillin Streptomycin (PS), 20 ng/ml Epidermal Growth Factor (EGF), .05 μg/ml Hydrocortisone, 10 μg/ml Insulin-bovine, 100 ng/ml Cholera Toxin
AU565	Human Breast Cancer HER2+/ER-	Roswell Park Memorial Institute-1640 Medium (RPMI), 10%Fetal Bovine Serum (FBS), 1%Ps
MCF7	Human Breast Cancer HER2-/ER+	Minimum Essential Media (MEM)α, 10%FBS, 1%PS, 0.01 mg/ml Insulin- bovine
MDA- MB231	Human Breast Cancer HER2+/ER2+	DMEM, 10%FBS, 1% PS
hDFB	Human Dermal Fibroblasts	DMEM, 10%FBS, 1%PS
NHA	Normal Human Astrocytes	NHA media from Lonza
U118MG	Human Glioblastoma	DMEM, 10% FBS, 1%PS
NHOst	Normal Human Osteoblast	NHOst media from Lonza
MG63	Human Osteosarcoma	DMEM, 10%FBS, 1%PS
RWPE-1	Non-tumorigenic Human Prostate	Keratinocyte serum free media from Gibco
DU145	Human Prostate Carcinoma	DMEM, 10%FBS, 1%PS

The eleven human cell lines and the corresponding culture media used in our experiments are listed in Table 1. These cells were chosen to represent connective, epithelial and neural tissue shown in Table 2. All cells were grown in conventional 2D cell culture flasks at 37°C in a humidified incubator containing 5% CO₂. Upon reaching 80% confluency, cells were collected by treatment with trypsin-EDTA (SAFC Biosciences), washed in phosphate buffered saline (PBS), counted with a hemocytometer, and suspended in 1% collagen solution (1 × 10⁶ cells/ml) to form hydrogels as described previously [14]. Gels were maintained under the same conditions as the 2D cultures.

ISSN: 1471-2342