Left ventricular segmentation from MRI datasets with edge modelling conditional random fields
- Janto F Dreijer^{1, 2}Email author,
- Ben M Herbst^{1} and
- Johan A du Preez^{2}
DOI: 10.1186/1471-2342-13-24
© Dreijer et al.; licensee BioMed Central Ltd. 2013
Received: 26 September 2012
Accepted: 25 July 2013
Published: 31 July 2013
Abstract
Background
This paper considers automatic segmentation of the left cardiac ventricle in short axis magnetic resonance images. Various aspects, such as the presence of papillary muscles near the endocardium border, makes simple threshold based segmentation difficult.
Methods
The endo- and epicardium are modelled as two series of radii which are inter-related using features describing shape and motion. Image features are derived from edge information from human annotated images. The features are combined within a discriminatively trained Conditional Random Field (CRF). Loopy belief propagation is used to infer segmentations when an unsegmented video sequence is given. Powell’s method is applied to find CRF parameters by minimizing the difference between ground truth annotations and the inferred contours. We also describe how the endocardium centre points are calculated from a single human-provided centre point in the first frame, through minimization of frame alignment error.
Results
We present and analyse the results of segmentation. The algorithm exhibits robustness against inclusion of the papillary muscles by integrating shape and motion information. Possible future improvements are identified.
Conclusions
The presented model integrates shape and motion information to segment the inner and outer contours in the presence of papillary muscles. On the Sunnybrook dataset we find an average Dice metric of 0.91±0.02 and 0.93±0.02 for the inner and outer segmentations, respectively. Particularly problematic are patients with hypertrophy where the blood pool disappears from view at end-systole.
Background
Manual annotation is a tedious process and lacks consistency between human annotators [1, 2] and even between separate annotations by the same annotator.
Various properties of magnetic resonance images, such as intensity inhomogeneities, z-shift due to respiration and imaging acquisition artifacts, can make segmentation difficult. One of the most severe problems arises from judging to what extent the papillary muscles influence and, possibly, obscure the endocardium border. For research on the effects that discrepancies in annotations of the papillary muscles can have on the calculation of left ventricle function and mass see e.g. [2–4]. In this work we primarily focus on mitigating the effect of the papillary muscles.
A number of automated techniques have been developed for the segmentation of the left ventricle from its surrounding structure (see e.g. [5, 6]). For a review of snakes and deformable models in medical image analysis see e.g. [7]. We will briefly discuss the most popular techniques.
Active Shape Models (ASMs, [8]) track the edges in a video sequence by modelling the contour shape using methods such as Principal Component Analysis. This is often combined with a Kalman filter to incorporate temporal smoothing in an online tracking framework. Typically only past information is used and future images ignored, often with adverse consequences if the object being tracked disappears from view or becomes very small.
Andreopoulos and Tsotsos [9] fit a 3D Active Appearance Model (AAM) and investigate a hierarchical “2D + time” ASM that integrates temporal constraints by using the third dimension for time instead of space.
Generative models such as Markov Random Fields (MRF) are popular in pixel labelling and de-noising problems [10]. Modelling the image probability can, however, lead to intractable models with complex dependencies between local features. This can lead to reduced performance if oversimplified [11]. Careful manual design of the probability distributions is therefore often necessary.
Most image segmentation applications of MRFs also model the texture within a region and are constructed to favour spatially smooth regions. We refer to these models as Surface MRFs. Surface MRFs are often used to isolate homogeneous objects from their backgrounds. The left ventricle, however, contains papillary muscles (see Figures 1 and 2), rendering this approach less effective. As surface MRFs do not model the edge explicitly, they do not directly encode any shape information. There have been attempts to unify models of the edge and surface: specifically, Huang et al. [12] propose coupling surface MRFs with a hidden state representing a deformable contour.
Cordero-Grande et al. [13] construct an MRF model of the inner and outer contours, using a purely generative model. They use the Gibbs sampling algorithm to extract segmentations and parameters.
Also of interest is the approach by Jolly [14], in which the segmentation problem is set in log-polar space where the least cost path in a single frame (calculated by dynamic programming) is defined as the desired contour. A cost function, which they relate to an initial labelling of blood pool pixels, is required to determine the correct contour. This is similar to our approach in that if we limit our model to a single frame (i.e. remove temporal linkage) belief propagation also reduces to estimating the least cost path via dynamic programming.
In summary, a number of methods are available, with the main challenge being integration of temporal and spatial constraints.
In an earlier study, Dreijer et al. [15] investigated modelling the endocardium edge using a semi-conditional MRF with mostly heuristically chosen features. Although this approach showed promise, practical experiments indicated a tendency to “snap” onto the epicardium, partly due to the epicardium’s stronger edge with respect to surrounding tissue. In the present study, the outer contour is explicitly included in the model, establishing a statistical dependency between the two contours. We also train discriminative feature functions from human annotated images as opposed to the heuristically chosen functions used previously.
Methods
Conditional random field
A Conditional Random Field (CRF, [16]) models the conditional probability of a set of unobserved/latent variables, y, given a set of observations x, i.e. P(y|x). This is different from a generative Markov random field which models the probability distribution of the observed variables, given different configuration of the latent variables, i.e. P(x|y).
where the normalizing partition function $Z={\sum}_{\mathit{y}}{\prod}_{c\subset \mathit{y}}{\psi}_{c}\left({\mathit{y}}_{c},\mathit{x}\right)$ sums over all possible configurations of y.
The main advantage of a CRF is its discriminative nature, i.e. it does not require a detailed model of the observed information, instead, computational resources are dedicated to describing the latent variables.
Problem formulation
We refer to the left ventricle endocardium as the inner contour and the left ventricle epicardium together with the right ventricle’s endocardium (bordering the septum) as the outer contour, see Figure 1.
We are primarily interested in segmenting a video sequence of T grayscale images, I(0),…,I(T − 1), that is synchronised with a single cardiac cycle so that the first image I(0) is before systole (contraction) and the last image, I(T − 1), after diastole (relaxation). End-systole (maximum contraction) thus occurs in the middle of the video sequence at approximately I(T/2). We refer to the grayscale value of a pixel within a single image at the x,y coordinate p as I(t,p).
where ⌊x⌋ is the floor function of x and r _{init}=50 is experimentally chosen such that, for most segmentations in the training set, ρ _{ n }(t)≈M/2 at end-diastole. The radius is discretised as ρ _{ n }∈{0,…,M − 1} where M=256 provides a resolution sufficient for human segmentation of the transformed image. One advantage of the log-space is that it provides a better spatial resolution at smaller radii.
Note that the position of the centre point is different for each frame. Partly due to the non-symmetrical contraction of the heart, the ventricle centre point can undergo significant translation.
Our segmentation process first determines a series of centre points, after which relative radial values are inferred. In the next section we turn our attention to finding suitable centre points.
Centre point estimation
Our CRF model requires the centre points to be placed near the middle of the inner contour allowing the model to restrict the spatial and temporal variability of the radii. As a semi-automatic clinical tool, a human operator could annotate the centre point in every frame of the video sequence. In our work towards a fully-automatic technique we describe a semi-automated procedure that requires only annotating the centre point in one frame, after which the other centre points are estimated. Fully-automatic techniques are especially valuable when analysing significantly large datasets.
A number of heuristic techniques (e.g. [5]) are available for estimating the centre points. Most of these perform adequately when the papillary muscles are absent and there is high contrast between the blood pool and cardiac wall. This is generally the case for the first few frames, but not at the end of systole when the ventricle is at its smallest.
The procedure described below requires the user to provide the centre point c(0) of the first frame when the left ventricle blood pool is clearly visible and the papillary muscles minimally obstruct the inner contour. The robustness against variations in c(0) is discussed in Section “Sensitivity to initial centre point placement”. With an appropriate user interface the annotation of the center point in the first frame of all spatial slices would only take the user a few seconds.
Since each video sequence from our dataset contains a single heart beat, and due to its periodic nature, we assume the last frame has the same centre point as the first, i.e. c(T − 1)=c(0). This is not a severe restriction since the algorithm is robust against variations in c(t), as demonstrated in Section “Sensitivity to initial centre point placement”.
where I ^{ c(t)}(t) is the image I(t) centred at c(t) such that I ^{ c(t)}(t,p)=I(t,p−c(t)) and zero when indexed out of bounds, p is, again, the x,y coordinates and I(t) the frame at time t before a log-polar transform is applied.
The weight ${w}^{c\left(t\right)}\left(\mathit{p}\right)={e}^{\left(-{\u2225\mathit{c}\left(t\right)-\mathit{p}\u2225}^{2}/{\sigma}^{2}\right)}$ locally enhances the error around the frame’s centre point. The width, σ, is experimentally chosen from a training dataset so that the inferred centre points closely resemble the mean of the ground truth inner contours. For computational efficiency we only calculate values within a 2σ radius of c(t) as the contribution becomes negligible further away.
Note that (7) is a nonlinear function of the sequence of centre points, but is efficiently solved using an optimization strategy such as dynamic programming. In addition, in order to reduce computational cost a beam search is implemented, effectively assuming the centre point translates less than three pixels between frames. Backtracking is initiated at the centre of the last frame c(T − 1). In order to constrain c(0) to the value provided by the user, during dynamic programming, the cumulative cost function for the first frame is set to zero at that value and 1 otherwise.
We pose the centre point estimation problem as one that can be solved within the same belief propagation framework as our radial inference. However, since this is independent of the radial inference fast heuristic methods could be considered in the future.
The CRF model
In this formulation, smaller energies indicate better segmentations, while bad segmentations are penalized with larger energies.
sums over all possible configurations of ρ, normalizing the exponentiated energy into a probability.
Note that there are no significant theoretical restrictions to the feature functions in a random field. Any further restrictions and choices of feature functions are design choices made to improve performance.
Feature functions
We restrict ourselves to positive feature functions f where small values are more desirable (i.e. smaller values should correlate with “better” segmentations). Positive parameters, θ, determine the relative weights of the feature functions. In order to minimize the clique size, we also restrict ourselves to features that couple at most two radial values in space and time, as computational cost grows exponentially with increasing clique size.
We derive the feature functions from discriminative properties of human annotated images as described below. Selection of the relative weights, θ, is discussed in Section “CRF parameter estimation”.
Feature function based on edge classifiers
For a log-polar frame (such as in Figure 4) at time t, consider a window of height 1 and width $w=\frac{M}{4}=64$ around a radius ρ in the radial direction n. This window is equivalent to a circular sector in the original image before the log-polar transform is applied. We refer to the pixel values in this window as the vector ${\mathit{v}}_{\rho}={\mathit{d}}_{n}\left(t,\left[\rho -\frac{w}{2},\dots ,\rho +\frac{w}{2}\right]\right)$. A feature vector κ(v) is derived from the window and is described below.
We train an artificial neural network, with two nodes in a hidden layer, to model the presence of the cardiac edge. A window extracted from the training set is considered to contain an edge if the centre of the window is no more than 2 radial distances away from the ground truth edge, otherwise it is considered a “non-edge” training example.
The expression $\left[\left|\frac{\partial \mathit{v}}{\mathit{\partial \rho}}\right|>\u03f5\right]$ is a binary value indicating the presence of a gradient.
From the short-axis view in Figure 1, it is worth noting that the gradient’s sign on the left and right sides of the outer contour’s edge differ, due to the intensity of the right ventricle’s blood pool. We therefore train eight classifiers for different parts of the contour, i.e. instead of training a single classifier over all angular directions (n=0..N−1) we treat groups of angles separately (n=0,…,15, n=16,…,31 etc.) and thus train direction dependent classifiers. This allows the classifiers to exploit features that it might find relevant in that direction.
We repeat the process for the classifiers of the inner contour’s edges as the endocardium’s edge behaviour also differs between the left and right sides of the ventricle due to the presence of the papillary muscles on mostly one side (see Figure 4).
Heiberg [6] identifies the edges of the inner and outer contours as two classes: Concordant, where edge gradients have a similar sign, and Discordant, areas where the edge gradient sign differ. Accordingly, he explicitly includes the gradient sign for the inner contour and ignores the sign for the outer. Because our edge model is trained on annotated ground truth images, our classifier automatically differentiates between the signs when they are relevant to edge detection.
To fit within the framework of energy minimization, the response of the neural network to an image is transformed into a cost by subtracting its output value from one. The minimum cost in the radial direction is subtracted to avoid negative feature values and is normalized by the sum.
We construct these networks for the inner and outer contour edges and so derive the features ${f}^{\text{in}}\left({\rho}_{n}^{\text{in}}\left(t\right),{\mathit{d}}_{n}\left(t\right)\right)$ and ${f}^{\text{out}}\left({\rho}_{n}^{\text{out}}\left(t\right),{\mathit{d}}_{n}\left(t\right)\right)$.
It should be noted that the typical neural network training techniques attempt to minimize a classification error, however we are not so much interested in the classification as using the output to penalise non-edges while at the same time minimally penalising edges. Effectively, we would prefer a classifier that rejects very few edges. To mitigate unintended penalisation, the outputs of the constructed features are “smoothed” by applying a small minimum-filter (erosion), in the angular direction. This will cause an area to have a high feature response (i.e. penalised) only if its neighbouring areas (in the angular direction) also have high responses.
Spatial and temporal feature functions
Figure 4 shows that there are strong gradients at some non-contour positions and relatively weak gradients at some contour positions, especially when papillary muscles are close to the endocardium border, resulting in the feature responses of Figures 6 and 7. This is undesirable as inference from these features alone would cause incorrect segmentations in these areas.
where M is again the discretisation value, used here to scale the feature values to the same order of magnitude as the features described previously. From direct inspection, few contours violate the properties |ρ _{ n }(t)−ρ _{ n }(t−1)|≤25 and |ρ _{ n }(t)−ρ _{ n−1}(t)|≤2. This can be exploited during inference by applying a beam search and thereby significantly reducing the search space.
For simplicity, we have chosen a fixed t _{ E S }=8 from inspection of the training annotations. The correct selection of t _{ E S } is sensitive to patient pathology. A more robust choice might depend on detecting when the optical flow in the images are suspended and reversed. A full discussion is, however, beyond the scope of this article. Again, these features are constructed separately for both the inner and outer contours.
where ϵ _{ ρ }=2 is an experimentally chosen radial offset. Similar feature functions are constructed for the intensity just outside the inner contour.
Inner-outer radius feature functions
Segmentation
Belief propagation is a popular technique for inferring values for the latent variables in a graphical model. Messages representing cumulative belief are passed between variables and updated according to a specific order or schedule. Backtracking is used to recover a solution. For a tutorial on belief propagation see e.g. [17]. When applied to chains or tree structures, belief propagation is equivalent to dynamic programming, however our model contains many loops and thus requires the iterative loopy belief propagation. For more detail, specifically as applied to this type of edge model, see e.g. [15]. we have chosen a sequential propagation schedule which allows for faster convergence.
Propagation schedule
In a parallel propagation schedule all messages are updated simultaneously after each iteration. However, in our experience, the effects from a feature function propagate relatively slowly through the model if a parallel schedule is followed. This is in line with Goldberger and Kfir [18]. For this reason we have chosen a sequential propagation schedule which allows for faster convergence.
For variables representing the inner contour, messages are first propagated in an angular direction (n=0,…,N−1) and reversed (n=N−1,…,0) before being propagated to the next temporal frame (t=0,…,T−1) and back (t=T−1,…,0). Similar steps are then repeated for the outer contour, taking into account messages passed from the inner contour. This process is repeated for three iterations.
Propagating over the angular direction first places more emphasis on contour continuity than the other radial relationships. The same reasoning is used in the selection of a backtracking order, as discussed below.
Message normalization
As features in an undirected graphical model are allowed to take on arbitrary values, propagated messages do not represent marginal probabilities, as is the case in directed graphs. If loopy belief propagation is applied without message normalization, numerical overflow can occur after only a few iterations.
We normalize our messages by subtracting the smallest value in each message before it is propagated, effectively assuring that the minimum value in each message is zero. For a max-product setting this is equivalent to normalizing each message so that the largest value in the message is one.
Convergence and optimality
We briefly discuss issues of convergence of message passing and on the choice of an appropriate backtracking order.
Belief propagation is guaranteed to yield a globally optimal result when applied to a tree structured graph [17]. It also converges to a stable fixed point (which is globally optimal) or periodically oscillates when applied to graphs with a single loop [19]. Due to the temporal and inner-outer radius feature functions our graphical model contains many loops (Figure 5).
Convergence for graphs with this many loops is not guaranteed, although if convergence is reached there are theoretical results regarding its optimality. Weiss and Freeman [19] describe the neighbourhood within which the result is optimal.
Through experimentation we find that the inferred segmentation is sensitive to the order of backtracking. We choose to backtrack over all nodes in the inner and outer contours independently. We find that this increases the probability that the inferred inner and outer contours in a single frame form continuous loops.
We have not observed divergence or significant oscillation between configurations in our application.
CRF parameter estimation
Maximum likelihood estimation
where D ^{(i)} is a video sequence from a training dataset and ρ ^{(i)} is its human annotated segmentation.
Here the sum is over all configurations of ρ which requires O(M ^{2N T }) operations. This is one of the significant challenges in applying CRF/MRFs to practical problems as the complexity quickly becomes intractable in general.
Attempts have been made by others to approximate the partition function [20, 21], which implies an approximation to the original distribution P that we refer to as the distribution $\stackrel{~}{P}$.
Apart from these computational difficulties, also note that the approximate nature of loopy belief propagation causes inference to yield a segmentation that is the optimal configuration to a different probability distribution (that we refer to as Q) than the one described in Section “The CRF model” (referred to as P). The relationship between these distributions is discussed by Weiss and Freeman [19].
The effect of using parameters that maximize the likelihood of the training data under $\stackrel{~}{P}$, to infer values from the distribution Q is unclear.
However, since we are primarily interested in obtaining parameters that yield good segmentations under inference and less with estimating the “true” model distribution, we investigate an alternative that avoids calculating the partition function.
Parameter estimation
Consider a video sequence D ^{(i)} from a training dataset and its human annotated segmentation ρ ^{(i)}. We are interested in obtaining parameters that would lead to a segmentation, ρ ^{⋆(i)}, of the video sequence, that does not significantly differ from the annotated segmentation, ρ ^{(i)}.
The function e _{ i } is a measure of the differences between two segmentations (i.e. the ground-truth and inferred contours). The landmark error, i.e. the average of the shortest distance between each point on the ground truth contour and the inferred contour, is used by Andreopoulos et al[9] in their evaluation. Due to its non-symmetry, i.e. e _{ i }(ρ ^{ A },ρ ^{ B })≠e _{ i }(ρ ^{ B },ρ ^{ A }), we have observed that inferred contours that minimize this error tend to be very jagged. For this reason we use the average of the “ground truth to inferred” error and the “inferred to ground truth” error during training, i.e. $\frac{{e}_{i}\left({\mathit{\rho}}^{A},{\mathit{\rho}}^{B}\right)+{e}_{i}\left({\mathit{\rho}}^{B},{\mathit{\rho}}^{A}\right)}{2}$.
Powell’s method [22] is a popular technique for searching for the local minimum of a function and is used here to find suitable parameters. Powell’s method avoids calculating a gradient through a bidirectional line search along a vector in a list of vectors. The list is updated by the displacement after each improving iteration. In our case convergence requires about 300 iterations.
To compensate for the approximate nature of inference and avoid calculating the partition function, a gradient-free approach is thus followed where the segmentation error is treated as a “black box”. This allows us to integrate the approximate inference process into the training stage.
Implementation
The majority of the software is implemented in the Python programming language with belief propagation implemented in C. Cordero-Grande et al. [13] reported segmentation of a single 4D video sequence in approximately 56 minutes on a single 800MHz CPU with 4MB cache from their MRF. For a similar number of images (12 slice positions with 20 frames each) on a single 3400MHz CPU with 8MB cache we can report a radial segmentation time of ~2 minutes. We have not included the time to estimate the centre point positions, which is approximately an additional minute for each 20 frame video sequence.
Our radial inference technique is thus approximately six times faster (taking into account the faster CPU), which can be attributed to their use of a Gibbs sampler. Note also that the number of radial points we use to represent a contour and its radial discretisation are 128 and 256, respectively. This is significantly larger than the values of 7 and 31 used by Cordero-Grande et al., especially when comparing the size of the resulting configuration space.
Results
In this section we analyse the segmentations produced by our process and compare them to those of a few existing techniques. The reader is urged to view the video sequences in Additional file 1 or on our website at http://dip.sun.ac.za/~janto. Additional file 2 contains the automatic segmentations of all end-systole and end-diastole frames for the Sunnybrook evaluation dataset.
We evaluate our model on two datasets. Our segmentation process is trained and analysed on the York dataset [9] with respect to segmentation behaviour and its sensitivity to placement of the initial centre point. This dataset contains ground truth annotations for all frames and therefore contains important temporal information. For comparison with other authors our technique is evaluated on the Sunnybrook [5] MRI dataset. This dataset is not annotated for all frames, but has the advantage that more authors have used this set to report their results.
In addition to the landmark distance, we use two other similarity measures that have gained widespread use: the Dice metric and Average Perpendicular Distance (APD). The Dice similarity represents the percentage of overlap between two segmented surfaces and the Dice error is the percentage of non-overlap, i.e. one minus the Dice similarity. The APD is calculated as the average of the perpendicular distance from each point on the reference contour to the target contour. The APD is therefore similar to the non-symmetric landmark distance.
York cardiac segmentation dataset
In this section we evaluate our model on the MRI York dataset [9] provided by the Department of Diagnostic Imaging of the Hospital for Sick Children in Toronto and annotated by Andreopoulos of York University. The dataset contains video sequences from 33 subjects, all under the age of 18, displaying a variety of heart abnormalities such as cardiomyopathy, aortic regurgitation, enlarged ventricles and ischemia. We split the dataset into three cross-validation sets: 11 subjects for training of the edge classifier, 11 subjects for CRF parameter estimation and 11 subjects for evaluation. Each set effectively contains approximately 100 video sequences and each video contains 20 frames at different z-axis slice positions. The inner and outer contours are manually annotated for all frames and are used as the ground truth in our experiments.
For efficiency we only use video sequences of the sixth z-axis slice of each patient to estimate the CRF parameters. These slices do not necessarily coincide with the mid ventricle for all patients. The number of slices where the ventricle is visible differs between patients and is likely dependent on patient pathology. A different choice of training data is thus likely to have a significant effect on the results of patients with, for example, hypertrophy.
Segmentation analysis
We also observe robustness to some noisy images. These examples suggest that in some of these images where the automatic segmentation differs from the ground truth, the automatic segmentation is superior to the manual approach. This is attributed to the fact that the automated system is able to integrate temporal behaviour, something that is an arduous task for a human.
Comparison of contour errors
Authors | Technique | Inner contour | Outer contour |
---|---|---|---|
error [mm] | error [mm] | ||
Our method | CRF | 1.57 | 1.78 |
Our method (without subjects 8 and 27) | CRF | 1.49 | 1.74 |
Andreopoulos and Tsotsos [9] | AAM | 1.43 | 1.51 |
Üzümcü [23] | Landmark tracking | 1.86 | 1.77 |
Jolly [14] | Shortest path | 2.44 | 2.05 |
Cordero-Grande et al. [13] | Edge MRF | 1.37 | 1.22 |
Lorenzo-Valdés et al. [24] | Surface MRF | 2.99 | 2.21 |
Table 1 contains segmentation errors of the inner and outer contours as reported by a selection of different authors. We report our results as the landmark error [9], i.e. the average of the shortest distances between the points on the ground truth contour and the inferred contour. These results are, however, from different datasets and there are also subtle, but important, differences in the error criteria. Refer to the individual papers for more detail. This table should therefore only be used as a rough comparison to other techniques. For a more comprehensive summary of reported errors see [25]. The Sunnybrook dataset is used in a later section to more fairly compare our technique to others.
Sensitivity to initial centre point placement
As can be seen from Figure 19, the segmentations of the inner and outer contours remain relatively stable while c(0) is within the inner 20% of the endocardium. When placed at approximately 50% between the ground truth centre point and inner contour, the spatial continuity assumption of (12) is violated enough that the quality of the inferred contours begin to deteriorate significantly.
Sunnybrook cardiac segmentation dataset
The Sunnybrook Cardiac MR Database [5] is provided by the Sunnybrook Health Sciences Centre and was used for the 2009 MICCAI Cardiac MR Left Ventricle Segmentation Challenge. The dataset contains 45 subjects, with an average age of 61, with diverse morphologies (Heart failure with and without infarction, LV hypertrophy, and healthy subjects) and is manually segmented by a cardiologist. The inner contours are annotated only at end-diastole and end-systole, while the outer contours are annotated only at end-systole. A ground truth segmentation of the intermittent frames is therefore not available, making extraction of temporal information for this dataset difficult.
Due to the sparsity of annotations in this dataset, all feature functions are re-used as derived from the York dataset. The model used to segment the Sunnybrook data therefore includes features derived from the trained edge classifiers, temporal behaviour and inner-outer relationships of the York dataset. Only the CRF parameters (i.e. the relative importance of the features) are retrained on a training subset of the Sunnybrook data. To further compensate for the relatively few examples, and thus avoid over-fitting, a very weakly weighted L1-norm parameter regularization term ($\sum _{d}\left|log{\mathit{\theta}}_{d}\right|$) is added to the objective function. Since this term is applied to the log of the parameters, it effectively penalizes the specialization on features by the optimizer. A detailed analysis of the effects of parameter regularization in our application is beyond the scope of this article.
This modification suggests that there are important model parameters that are dependant on the patient pathology. A practical segmentation tool could allow the operator the option to provide a prior diagnosis or more fine grained control over some settings.
During training of the edge classifier from the York dataset, it was assumed that the extracted radial window, v _{ ρ }, contains an edge if the annotated edge is within two radial distances from the middle of the window. This assumption is problematic if used to train the edge classifier on the Sunnybrook dataset. A small wall thickness, as is common in this dataset, would cause both the inner and outer contours to fall within this criteria, resulting in inconsistencies in the training examples and thus weakening the resulting edge detector. To train a classifier on this dataset it would thus be necessary to be more strict with regard to the minimum radial distance.
Segmentation analysis
Comparison of Sunnybrook Dice errors
Authors | Dice similarity | APD (mm) | ||
---|---|---|---|---|
Inner | Outer | Inner | Outer | |
Our method (trained on York) | 0.87 | 0.92 | 2.70 | 2.23 |
Our method (after retraining) | 0.91 | 0.93 | 1.84 | 1.95 |
Marak et al. [26] | 0.86 | 0.93 | 2.6 | 3.0 |
Lu et al. [27] | 0.89 | 0.94 | 2.07 | 1.91 |
Wijnhout et al. [28] | 0.89 | 0.93 | 2.29 | 2.28 |
Casta et al. [29] | - | 0.93 | - | 2.72 |
O’Brien et al. [30] | 0.81 | 0.91 | 3.73 | 3.16 |
Constantinides et al. [31] | 0.89 | 0.92 | 2.35 | 2.04 |
Huang S. et al. [32] | 0.89 | 0.94 | 2.10 | 1.95 |
Jolly [14] | 0.88 | 0.93 | 2.44 | 2.05 |
Sunnybrook results
Patient | Good (%) | APD (mm) | Dice similarity | |||
---|---|---|---|---|---|---|
Inner | Outer | Inner | Outer | Inner | Outer | |
SC-HF-I-05 | 100 | 100 | 1.52 | 1.86 | 0.94 | 0.95 |
SC-HF-I-06 | 100 | 100 | 1.66 | 1.45 | 0.92 | 0.95 |
SC-HF-I-07 | 100 | 100 | 2.37 | 2.79 | 0.89 | 0.90 |
SC-HF-I-08 | 95 | 100 | 1.68 | 1.37 | 0.93 | 0.96 |
SC-HF-NI-07 | 100 | 100 | 2.21 | 2.20 | 0.91 | 0.93 |
SC-HF-NI-11 | 100 | 100 | 1.70 | 1.25 | 0.93 | 0.96 |
SC-HF-NI-31 | 100 | 100 | 2.06 | 1.58 | 0.91 | 0.95 |
SC-HF-NI-33 | 94 | 100 | 1.68 | 1.64 | 0.91 | 0.94 |
SC-HYP-06 | 92 | 100 | 1.67 | 2.05 | 0.90 | 0.92 |
SC-HYP-07 | 69 | 100 | 1.39 | 1.83 | 0.93 | 0.94 |
SC-HYP-08 | 68 | 100 | 2.27 | 2.33 | 0.90 | 0.93 |
SC-HYP-37 | 69 | 71 | 2.21 | 2.44 | 0.86 | 0.91 |
SC-N-05 | 93 | 100 | 1.67 | 2.45 | 0.89 | 0.89 |
SC-N-06 | 100 | 86 | 1.76 | 2.12 | 0.89 | 0.91 |
SC-N-07 | 100 | 100 | 1.79 | 1.95 | 0.88 | 0.90 |
mean | 92 | 97 | 1.84 | 1.95 | 0.91 | 0.93 |
std deviation | 12.4 | 8.0 | 0.30 | 0.44 | 0.02 | 0.02 |
Discussion
In this article, the CRF parameters are estimated using a gradient-free search approach. The time spent searching for parameters can be further reduced by considering only a subset of the training videos. Due to the relatively low dimensionality of the parameter search space (≈14 dimensions) and relatively informative nature of each video sequence for all parameters, data scarcity is not a problem. It is, however, advisable that the training set contains a sufficient number of video sequences in which the papillary muscles obscure the endocardium border.
Note that no image preprocessing is done to compensate for effects such as different intensity settings on the MRI equipment. Image equalisation before segmentation would likely result in more robust result.
Inclusion of spatially neighbouring slices into a unified 3D and time model might also increase segmentation accuracy. Information at spatially higher slices, where the papillary muscles are less problematic, could then be used to improve the accuracy at lower slices. Linking the radial values between different slices would be relatively simple. This can be done with appropriate feature functions similar to those restricting radial continuity in a single contour. This would, however, require aligning slices to compensate for translation caused by different levels of inhalation and expiration between slices. It should also be simple to extend the segmentation process to include these additional features by adding them to the propagated messages.
Ideally, the time of end-systole in (14) should not be decided before inference, however, this might require a second order CRF or modelling as an additional unobserved variable. This would lead to an increase in segmentation time. Alternatively, techniques based on detecting temporary suspension and reversal of optical flow could be useful in detecting end-systole.
A second order system would also make the incorporation of contour smoothness information possible, as currently only contour continuity is taken into account. Alternatively, post-processing of the resultant contours by fitting them to splines, would improve smoothness.
From a visual inspection of the ground truth it is clear that there are inconsistencies in the human annotations with regard to the inclusion of the papillary muscles in the inner contour. These inconsistencies reduce the discriminative ability of the edge classifier and influences the optimal CRF parameter values estimated during training. Also, because the human annotated contours are used for evaluation, inconsistencies of the human annotations need to be taken into account when interpreting any results based on this as ground truth. In short: inconsistent examples in the training and evaluation set will result in an upper limit to the accuracy achievable by any consistent system.
Conclusion
We present a CRF implementation for the automated segmentation of the left ventricle. Features are derived from discriminative properties of a human annotated dataset. The algorithm exhibits robustness against inclusion of the papillary muscles by integrating shape and motion information.
Experiments on the Sunnybrook dataset suggests that our technique would provide segmentations superior to those reported in the challenge.
The most significant segmentation errors are present in images of patients with hypertrophy, when the blood pool disappears from view. This limitation is due to the assumption that the inner contour is present in each frame. Future work could address these failures, possibly through the modelling of the right ventricle’s center point to avoid the LV outer contour from snapping to the outer edge of the entire heart structure.
Additional modelling of the right ventricle would also be beneficial, but is complicated by the polar coordinate space formulation, which allows only for one center point per frame.
Future work could also include faster and more robust centre point estimation. As mentioned previously, framing the centre point estimation in a model that can be solved with dynamic programming allows us to formulate its optimization as a belief propagation algorithm. This has the advantage that the centre point can be estimated as an additional latent variable in our CRF model, that needs to be inferred. Alternating between inferring ρ and c is thus theoretically possible, although insufficient research has been done as to its practical value.
Declarations
Authors’ Affiliations
References
- Anderson JL, Weaver AN, Horne BD, Jones HU, Jelaco GK, Cha JA, Busto HE, Hall J, Walker K, Blatter DD: Normal cardiac magnetic resonance measurements and interobserver discrepancies in volumes and mass using the papillary muscle inclusion method. Open Gen Intern Med J. 2007, 1: 6-12.View ArticleGoogle Scholar
- Janik M, Cham MD, Ross MI, Wang Y, Codella N, Min JK, Prince MR, Manoushagian S, Okin PM, Devereux RB, Weinsaft JW: Effects of papillary muscles and trabeculae on left ventricular quantification: increased impact of methodological variability in patients with left ventricular hypertrophy. J Hypertens. 2008, 26 (8): 1677-1685. 10.1097/HJH.0b013e328302ca14.View ArticlePubMedGoogle Scholar
- Burkhard Sievers M, Kirchberg S, Bakan A, Ulrich Franken M, Hans-Joachim Trappe M: Impact of papillary muscles in ventricular volume and ejection fraction assessment by cardiovascular magnetic resonance. J Cardiovasc Magn Reson. 2004, 6: 9-16.View ArticlePubMedGoogle Scholar
- Vogel-Claussen J, Finn J, Gomes A, Hundley G, Jerosch-Herold M, Pearson G, Sinha S, Lima J, Bluemke D: Left ventricular papillary muscle mass:relationship to left ventricular mass and volumes by magnetic resonance imaging. J Comput Assist Tomogr. 2006, 30 (3): 426-432. 10.1097/00004728-200605000-00013.View ArticlePubMedGoogle Scholar
- Radau P, Lu Y, Connelly K, Paul G, Dick A, Wright G: Evaluation framework for algorithms segmenting short axis cardiac MRI. MIDAS J - Card MR Left Ventricle Segmentation Challenge. 2009, [Sunnybrook Hospital]http://hdl.handle.net/10380/3070 Google Scholar
- Heiberg E: Automated feature detection in multidimensional images. PhD thesis,. Linkoping University, Sweden, 2004Google Scholar
- Mcinerney T, Terzopoulos D: Deformable models in medical image analysis: A survey. Med Image Anal. 1996, 1: 91-108. 10.1016/S1361-8415(96)80007-7.View ArticlePubMedGoogle Scholar
- Cootes T, Taylor C, Cooper D, Graham J: Active shape models - their training and application. Comput Vis Image Unders. 1995, 61: 38-59. 10.1006/cviu.1995.1004.View ArticleGoogle Scholar
- Andreopoulos A, Tsotsos JK: Efficient and generalizable statistical models of shape and appearance for analysis of cardiac MRI. Med Image Anal. 2008, 12 (3): 335-357. 10.1016/j.media.2007.12.003.View ArticlePubMedGoogle Scholar
- Li SZ: Markov, Random Field Modeling in Image Analysis. 2001, Secaucus: Springer-Verlag New York, Inc.View ArticleGoogle Scholar
- Sutton C, McCallum A, et al: Introduction to Statistical Relational Learning, An Introduction to Conditional Random Fields for Relational Learning. 2007, Cambridge: MIT Press, chap. 4Google Scholar
- Huang R, Pavlovic V, Metaxas DN: A graphical model framework for coupling MRFs and deformable models. Proceedings of CVPR 2004. 2004, Washington: IEEE Computer Society, 739-746.Google Scholar
- Cordero-Grande L, Vegas-Sánchez-Ferrero G, de-la Higuera PC, San-Román-Calvar JA, Revilla-Orodea A, Martín-Fernández M, Alberola-López C: Unsupervised 4D myocardium segmentation with a Markov Random Field based deformable model. Med Image Anal. 2011, 15 (3): 283-301. 10.1016/j.media.2011.01.002.View ArticlePubMedGoogle Scholar
- Jolly M: Fully automatic left ventricle segmentation in cardiac cine MR images using registration and minimum surfaces. MIDAS J - Card MR Left Ventricle Segmentation Challenge. 2009, [http://hdl.handle.net/10380/3114]Google Scholar
- Dreijer J, Du Preez J, Herbst B: Edge modelling MRFs for cardiac MRI segmentation. Pattern Recognit Assoc S Afr, Proc. 2010, 21: 81-86.Google Scholar
- Lafferty J, McCallum A, Pereira F: Conditional random fields: Probabilistic models for segmenting and labeling sequence data. Proceedings of the Eighteenth International Conference on Machine Learning. International Conference on Machine Learning. 2001, Morgan Kaufmann Publishers Inc: San Francisco, 282-289.Google Scholar
- Kschischang F, Frey B, Loeliger HA: Factor graphs and the sum-product algorithm. Inf Theory, IEEE Trans. 2001, 47 (2): 498-519. 10.1109/18.910572.View ArticleGoogle Scholar
- Goldberger J, Kfir H: Serial schedules for belief-propagation: analysis of convergence time. Inf Theory, IEEE Trans. 2008, 54 (3): 1316-1319.View ArticleGoogle Scholar
- Weiss Y, Freeman WT: On the optimality of solutions of the max-product belief propagation algorithm in arbitrary graphs. Inf Theory, IEEE Trans. 2001, 47 (2): 723-735. 10.1109/18.910584.View ArticleGoogle Scholar
- Besag J: Statistical analysis of non-lattice data. Statistician. 1975, 24 (3): 179-195. 10.2307/2987782.View ArticleGoogle Scholar
- Sutton C, McCallum A: Piecewise pseudolikelihood for efficient CRF training. International Conference on Machine Learning. 2007, NY: ACM Press, 863-870.Google Scholar
- Powell MJD: An efficient method for finding the minimum of a function of several variables without calculating derivatives. Comput J. 1964, 7 (2): 155-162. 10.1093/comjnl/7.2.155. [http://comjnl.oxfordjournals.org/content/7/2/155.abstract]View ArticleGoogle Scholar
- Üzümcü M: Constrained segmentation of cardiac MR image sequences. PhD thesis. Leiden University, Germany, 2007Google Scholar
- Lorenzo-Valdés M, Sanchez-Ortiz GI, Elkington AG, Mohiaddin RH, Rueckert D: Segmentation of 4D cardiac MR images using a probabilistic atlas and the EM algorithm. Med Image Anal. 2004, 8 (3): 255-265. 10.1016/j.media.2004.06.005. http://www.sciencedirect.com/science/article/pii/S1361841504000271,View ArticlePubMedGoogle Scholar
- Petitjean C, Dacher JN: A review of segmentation methods in short axis cardiac MR images. Med Image Anal. 2011, 15 (2): 169-184. 10.1016/j.media.2010.12.004. http://www.sciencedirect.com/science/article/pii/S1361841510001349,View ArticlePubMedGoogle Scholar
- Marak L, Cousty J, Najman L, Talbot H: 4D Morphological segmentation and the MICCAI LV-segmentation grand challenge. MIDAS J - Card MR Left Ventricle Segmentation Challenge. 2009, http://hdl.handle.net/10380/3085,Google Scholar
- Lu Y, Radau P, Connelly K, Dick A, Wright G: Automatic image-driven segmentation of left ventricle in cardiac cine MRI. MIDAS J - Cardiac MR Left Ventricle Segmentation Challenge. 2009, http://hdl.handle.net/10380/3109,Google Scholar
- Wijnhout J, Hendriksen D, Assen HV, der Geest RV: LV challenge LKEB contribution: fully automated myocardial contour detection. MIDAS J - Card MR Left Ventricle Segmentation Challenge. 2009, http://hdl.handle.net/10380/3115,Google Scholar
- Casta C, Clarysse P, Schaerer J, Pousin J: Evaluation of the dynamic deformable elastic template model for the segmentation of the heart in MRI sequences. MIDAS J - Card MR Left Ventricle Segmentation Challenge. 2009, http://hdl.handle.net/10380/3072,Google Scholar
- O’Brien S, Ghita O, Whelan P: Segmenting the left ventricle in 3D using a coupled ASM and a learned non-rigid spatial model. MIDAS J - Card MR Left Ventricle Segmentation Challenge. 2009, http://hdl.handle.net/10380/3110,Google Scholar
- Constantinides C, Chenoune Y, Kachenoura N, Roullot E, Mousseaux E, Herment A, Frouin F: Semi-automated cardiac segmentation on cine magnetic resonance images using GVF-Snake deformable models. MIDAS J - Card MR Left Ventricle Segmentation Challenge. 2009, http://hdl.handle.net/10380/3108,Google Scholar
- Huang S, Liu J, Lee LC, Venkatesh SK, Teo LLS, Au C, Nowinski WL: Segmentation of the left ventricle from cine MR images using a comprehensive approach. MIDAS J - Card MR Left Ventricle Segmentation Challenge. 2009, http://hdl.handle.net/10380/3121,Google Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2342/13/24/prepub
Pre-publication history
Copyright
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.