Three-dimensional evaluation of soft tissues in hyperdivergent skeletal class II females in Guangdong

Objectives To establish the three-dimensional facial soft tissue morphology of adolescent and adult females in the Guangdong population and to study the morphological characteristics of hyperdivergent skeletal class II females in Guangdong compared with that of normodivergent class I groups. Materials and methods The 3dMDface system was used to capture face scans of 160 patients, including 45 normal and 35 hyperdivergent skeletal class II adolescents (aged 11–14 years old) and 45 normal and 35 hyperdivergent skeletal class II adults (aged 18–30 years old). Thirty-two soft tissue landmarks were mapped, and 21 linear, 10 angular and 17 ratio measurements were obtained by 3dMDvultus analysis software. Data were assessed with a t-test of two independent samples between the normal adolescent and adult groups and between the normal and hyperdivergent skeletal class II groups. Results The linear measurements of the Guangdong adult females were larger than those of the adolescents in both Class I and Class II groups. However, the angular and ratio measurements had no significant difference. The vertical linear measurements were higher and the sagittal and transverse linear measurements were smaller in the hyperdivergent class II group (p < 0.05). The soft tissue ANB angle, chin-lip angle, and mandibular angle were significantly larger and the soft tissue facial convexity angle and nasal convexity angle were significantly smaller in the hyperdivergent class II group (p < 0.05). Additionally, there were significant differences in the ratio measurements between the hyperdivergent class II groups and the control groups (p < 0.05). Conclusions The three-dimensional facial morphology of Guangdong adolescent and adult females was acquired. The facial soft tissue measurements of the adults were higher in the three dimensions except for the facial convexity and proportional relationships which were similar, suggesting that the growth pattern remained the same. The three-dimensional facial soft tissue features of hyperdivergent skeletal class II were characterized by the terms “long, convex, and narrow”. Three-dimensional facial measurements can reflect intrinsic hard tissue characteristics. Supplementary Information The online version contains supplementary material available at 10.1186/s12880-022-00782-w.


Background
In recent years, more patients have sought orthodontic treatment to improve their facial aesthetics, and the majority have been female patients. Class II malocclusion has a high prevelance of 9.91% in Chinese schoolchildren [1]. Among the Guangdong population, skeletal class II malocclusion is quite common, and the chief complaint of these patients is facial protrusion [2]. Hyperdivergent skeletal class II malocclusion has obvious inharmonious jaw relationships in the sagittal, vertical and transverse directions. Fixing the facial soft tissue profile is the main treatment goal. The mechanism of vertical imbalance is complex. The growth pattern of the mandibular condyles with high-angle malocclusion is a backward growth, and the vertical growth type is expressed in the chin [3]. Clockwise rotation of the lower jaw due to the growth difference of anterior and posterior facial height is another important cause of the high-angle type [3]. These characteristics not only affect the patients' appearance but also cause psychological and mental disorders [4].
Vertical problems affecting the sagittal direction and facial protrusion are the key challenges in the orthodontic treatment of hyperdivergent skeletal class II malocclusion [3]. Clinical treatment for hyperdivergent class II malocclusion is complex and involves extensive efforts to control vertical aspects while solving sagittal and horizontal problems and avoiding worsening the facial aesthetics. Orthodontic treatments changes hard tissues in three dimensions, followed by soft tissue changes. However, the changes in soft tissue and hard tissue might differ during treatment. The improvement of soft tissue can provide a visual impression of the treatment results to patients. Therefore, we should fully understand both soft and hard tissue facial features in three dimensions to accurately carry out the diagnostic analysis, treatment plan and result evaluation.
Traditional tools for facial soft tissue analysis are digital photos and frontal and lateral cephalometric radiographs [5]. However, limited information is obtained from these 2-D images. The accuracy and repeatability of these images are affected by the patients' head position, camera angle, distance and so on. Three dimensional tools like cone beam computed tomography (CBCT) could overcome the drawbacks of 2-D images, but CBCT has radiation [6,7]. Recently, three-dimensional surface imaging technology has been used to obtain three-dimensional facial soft tissue information, reveal facial soft tissue more intuitively, and make accurate measurements [8]. 3dMDface System (3dMD LLC, Atlanta, Ga.) is a 3D imaging device with high reproducibility and accuracy [9]. It can provide a basis for comprehensive diagnosis and treatment planning, with a wide range of clinical applications [10][11][12]. However, the three-dimensional facial soft tissue morphology of adolescent and adult females of the Guangdong population has not been well studied. Soft tissue changes are one of the patients' objective requirements; therefore, research on soft tissue characteristics could aid in making comprehensive treatment plans. Few reports have examined the three-dimensional features of facial soft tissue in patients with hyperdivergent skeletal class II in Guangdong. In this study, we established a database of three-dimensional soft tissue characteristics of adolescent and adult hyperdivergent skeletal class II female patients compared to the characteristics of normal groups in Guangdong to treat this group of patients better, to lay the foundation for subsequent research, and to study the facial soft tissue characteristics of hyperdivergent skeletal class II female patients.

Subjects
This study was approved by the Ethics Committee of Sun Yat-sen University. A total of 160 subjects were included in this study. All the subjects were selected from the patients who had treatment in dental hospital. 3-D photos were captured before their treatment. Guangdong population was confirmed via a self-administered questionnaire. The inclusion criteria of this study are shown in Table 1. The different groups were classified according to the reference range from Steiner's analysis of Chinese population [13]. The exclusion criteria were facial asymmetry, previous orthodontic history, facial trauma or surgery.

3dMD images
The three-dimensional images were captured by a 3dMDface System (3dMD LLC, Atlanta, Ga.) and analyzed by 3dMDvultus software (3dMD LLC, Atlanta, Ga.) (Fig. 1). The 3dMD scanner had no radiation. The patient sat on an adjustable chair, and the distance between the subject and scanner was 1000 ~ 1100 mm. Before capturing the images, the instrument was calibrated according to the operating instructions. The system could automatically focus and capture a facial image of 180° between the ears. The capture speed was 1.5 ms [14]. The patient was seated in a relaxed, natural state, with both eyes looking straight ahead and a relaxed facial expression, keeping the posterior teeth at the maximum staggered position after swallowing, and the upper and lower lips closed gently. The data were stored in OBJ format files and imported into 3dMDvultus analysis software for further analysis.

Parameters measured
3dMDvultus analysis software was applied to locate 32 soft tissue landmarks (Fig. 2) to calculate 21 linear, 10 angular and 17 ratio measurements (Tables 2, detailed measurement definition are seen in the Additional file 1). The three-dimensional facial soft tissue differences of normodivergent skeletal class I adolescent females were analyzed and compared with that of normodivergent skeletal class I adult females. 3D facial soft tissue discrepancies of the adolescents/adults with hyperdivergent skeletal class II malocclusion were analyzed and compared with that of normodivergent skeletal class I group.

Statistical analysis
The operator was strictly trained to locate landmarks to ensure the landmarking process was accurate and consistent. All measurements in this study were performed by the same operator in a continuous period under the same conditions. If the difference was greater than 0.5 mm, the operator analyzed the reason and then reperformed the measurement until the distance between the two fixed points was not greater than 0.5 mm. This study used SPSS 20.0 software (IBM Corp., USA) for statistical analysis. Descriptive statistics of the measured data were acquired, and all statistical data are expressed as the mean ± standard deviation (x ± SD). A normal distribution of our data was shown using the normality test. A t-test of two independent samples was performed on the data of normodivergent skeletal class I adolescent and adult females and on the data of adolescents/adults with hyperdivergent skeletal class II malocclusion and the corresponding control group. α = 0.05 and p < 0.05 were considered statistically significant.

Comparison of linear measurements between normodivergent skeletal class I adolescent and adult females
There were significant differences between these groups. In the vertical direction, the anterior facial height, anterior upper facial height and posterior facial height of the adult group were larger than those of the adolescent group (p < 0.05). Although the anterior forehead height, anterior lower facial height, lip height, mandibular height and chin height were larger than those of the adolescent group, the difference was not statistically significant.
In the sagittal direction, the facial depth and mandible length in the adult group were larger than those in the adolescent group (p < 0.05). In the transverse direction, the facial width, mandibular width, interzygomatic width, buccal width, outer canthic diameter, lip width and nasal width of the adult group were larger than those of the adolescent group (p < 0.05). In summary, the facial threedimensional distance measurements of the normodivergent skeletal class I adult female patients were larger than those of the adolescent group (Table 3).

Comparison of angular measurements between normodivergent skeletal class I adolescent and adult females
There were no significant differences in the angular measurements between these two groups ( Table 4).

Comparison of ratio measurements between normodivergent skeletal class I adolescent and adult females
The lip width/lip height ratio and mandibular width/ facial width ratio of the adult group were larger than those of the adolescent group, and the differences were statistically significant (p < 0.05). The outer canthic diameter/mandibular width of the adult group was smaller than that of the adolescent group, and the differences were statistically significant (p < 0.05). No significant differences in other ratio measurements were seen between these two groups ( Table 5).

Comparison of linear measurements between adolescent females with hyperdivergent skeletal class II and those with normodivergent skeletal class I malocclusion
There were significant differences between the two groups. In the vertical direction, the anterior lower facial height and mandibular height of Class II group were larger, the posterior facial height of Class II group was smaller than that of control group, and the differences were statistically significant (p < 0.05). In the sagittal direction, the mandible length of the Class II group was smaller than that of the control group, and the differences were statistically significant (p < 0.05). In the transverse direction, the mandibular width, interzygomatic width and buccal width were smaller than those of the control group, and the differences were statistically significant (p < 0.05) ( Table 6).

Comparison of angular measurements between adolescent females with hyperdivergent skeletal class II and those with normodivergent skeletal class I malocclusion
The soft tissue facial convexity angle and nasal convexity angle of the Class II group were smaller than those of the control group, and the differences were statistically significant (p < 0.05). The soft tissue ANB angle, chin-lip angle, and mandibular angle of the Class II group were larger than those of the control group, and the differences were statistically significant (p < 0.05) ( Table 7).

Comparison of ratio measurements between adolescent females with hyperdivergent skeletal class II and those with normodivergent skeletal class I malocclusion
The anterior lower facial height/anterior facial height ratio, mandibular height/anterior facial height ratio, anterior facial height/posterior facial height ratio, anterior upper facial height/posterior facial height ratio, and anterior lower facial height/posterior facial height ratio of the Class II group were larger than those of the control group, and the differences were statistically significant (p < 0.05). The outer canthic diameter/facial  width ratio and outer canthic diameter/mandibular width ratio of the Class II group were larger than those of the control group; the mandibular width/facial width of the Class II group was smaller than that of the control group (p < 0.05) ( Table 8).

Comparison of linear measurements between adult females with hyperdivergent skeletal class II and those with normodivergent skeletal class I malocclusion
In the vertical direction, the anterior facial height, anterior lower facial height, mandibular height, and chin height of the hyperdivergent skeletal class II group were greater than those of the control group (p < 0.05). In the sagittal direction, the left and right posterior facial height, left and right facial depth, and left and right mandibular body length were smaller than those of the control group. (p < 0.05). In the transverse direction, the facial width, mandibular width, interzygomatic width, buccal width, inner canthic diameter and nasal width were lower in the hyperdivergent skeletal class II group than in the control group (p < 0.05) ( Table 9).

Comparison of angular measurements between adult females with hyperdivergent skeletal class II and those with normodivergent skeletal class I malocclusion
The soft tissue facial convexity angle and nasal convexity angle of the hyperdivergent skeletal class II adult female group in Guangdong were smaller than those of the control group. The soft tissue ANB angle, chin-lip angle, and left and right mandibular angle were larger than those of the control group, and the difference was statistically significant (p < 0.05) ( Table 10).

Comparison of ratio measurements between adult females with hyperdivergent skeletal class II and those with normodivergent skeletal class I malocclusion
There were significant differences in the facial soft tissue ratio measurements between hyperdivergent skeletal class II adult females and the corresponding normodivergent skeletal class I group in Guangdong. The anterior lower facial height/anterior facial height ratio, mandibular height/anterior facial height ratio, chin height/anterior facial height ratio, anterior facial height/posterior facial height ratio, anterior upper facial height//posterior facial height ratio, anterior facial height/posterior facial height ratio, and outer canthic diameter/mandibular width of the hyperdivergent skeletal class II group were significantly greater than those of the control group (p < 0.05). The anterior upper facial height/anterior lower facial height ratio, lip width/lip height ratio, and mandibular width/facial width ratio of the hyperdivergent skeletal class II group were smaller than those of the control group, and the difference was statistically significant (p < 0.05) (Table 11).

Discussion
Patients of different races, regions, genders, ages, and malocclusion types have different soft and hard tissues [15][16][17][18]. If different population standards are directly applied to the evaluation of the Chinese population, the results could be biased. Bishara et al. studied the longitudinal changes in the facial soft tissue protrusions of 35 subjects from 5 to 45 years old and found that facial protrusion of men and women showed a decreasing trend with age [19]. Therefore, we need to establish reference standards for the corresponding races, regions, genders, ages, and malocclusion types to better serve the local population.
The peak of female facial changes occurs in years 10-15 [19]. The 11-to 14-year-old females selected in this study were in a rapid growth change period. There were significant differences compared to adulthood, indicating that there was rapid growth in the three-dimensional facial linear measurements consistent with physiological age. The adult females' anterior facial height, anterior upper facial height, facial depth, mandible length, facial width, mandibular width, intercondylar width, buccal width, outer canthic diameter, lip width and nasal width were larger than those of adolescents, indicating that the soft tissue development of 11-to 14-year-old adolescents has great growth potential with a higher, deeper and wider tendency. Angular measurements can reflect the relative protrusion of each part of the face. Our data revealed that the normodivergent skeletal class I adolescent and adult females had similar relative protrusions of the various parts of the face despite their age difference.   [20,21]. Most of the proportional measurements were similar in adolescent and adult groups, suggesting that from adolescence to adulthood, their growth patterns remained unchanged. The ratio of lip width/lip height in the adult group is larger than that in the adolescent group, which may be due to the lip thinning with age; the ratio of outer canthic diameter/mandibular width in the adult group is significantly smaller than that of adolescent group, and the ratio of mandibular width/facial width in the adult group is larger than that of adolescent group. The width of the mandible is still increasing due to growth and development. Palomo et al. [22] found that compared with Class I girls, Class II girls had a longer facial pattern and more protrusive maxilla. The three-dimensional facial soft tissue measurements of adolescent females in Guangdong can reflect their distinctive characteristics of hyperdivergent skeletal class II, showing "long, convex, narrow" characteristics. The facial protrusion angle and nasal protrusion angle of hyperdivergent class II were smaller than those of the control group, and the soft tissue ANB angle and the chin-lip angle were significantly larger than those of the control group. The mandible is in the distal position relative to the forehead, nose, maxilla and upper lip, which reflects the problem of poor lower jaw development. The ratio measurement results may be due to the clockwise rotation of the mandible of the hyperdivergent patients or insufficient development of the mandibular ramus, which results in an increase in the height of the facial lower part and an increase in the ratio of the anterior height/posterior height. The soft tissue also showed a vertical growth pattern consistent with that of hard tissue.