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Fig. 4 | BMC Medical Imaging

Fig. 4

From: Analysis of vascularization in thyroid gland nodes with superb microvascular imaging (SMI) and CD34 expression histology: a pilot study

Fig. 4

User interface of the image processing program, ImageJ, for evaluating CD34-labelled tissue sections. a An extracted image shows the fast-red-stained areas; a rectangular area (yellow box) is selected for analysis. b The threshold function renders the display to show c only image areas with a positive pixel value (black). Then, the measure key is used to evaluate the area of the CD34-positive portion (black portions of the right image) within the selection area (yellow rectangle), which is displayed in the Results window (bottom)

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