Fig. 6From: The use of matrigel has no influence on tumor development or PET imaging in FaDu human head and neck cancer xenograftsImmunohistochemistry (IHC) sections of micro vessel density (CD31 staining), proliferating cells (Ki-67 staining), and non-viable cell count (HE staining). Selection of IHC results presented to illustrate collected tissue samples and analyzed tissue. a CD31 staining visualizing endothelial cells in vessels of tumor slide. Staining made with antibody concentration of 1:50. Picture originates from mouse in the −MG group. Arrows indicate positive staining of vessels in slide. b Edited version of picture A after using the CAIMAN segmentation algorithm to detect antibody stained areas. Arrows indicate vessels identified by the CAIMAN algorithm (marked by thin green contouring line). c Ki-67 staining for proliferating cells in tumor section from a mouse in the +MG group. Staining made using antibody concentration of 1:400. d Review of image C after using the ImmunoRatio application for selecting proliferating cell percentage. Cells marked in brown are considered as positive Ki-67 and blue cells negative. e Haematoxylin eosin staining for non-viable cell count in tumor slide from mouse in the +MG group. f Image E after separation of viable cells (red), non-viable cells (green), and background (purple) with the Advanced Weka Segmentation tool in FiJi (ImageJ)Back to article page