Fig. 5
Boxplot presentation of immunohistochemical results of collected tumors at different size stages. Results presented from all IHC data obtained. One way ANOVA with Holm-Sidak’s post hoc test. a MVD as determined by CD31 staining. Significance found between groups of sizes 500–700 mm3 (p-value = 0.0457) and 800–1100 mm3 (p-value = 0.0214). MVD calculated as no. of vessels per ROI area. b Average vessel area incl. lumen from CD31 staining. Significant difference between groups in tumors size of 500–700 mm3 (p-value = 0.024). c Non-viable cell count presented from HE staining of slides. No significance found. d Cell density calculated from Haematoxylin staining illustrated, no significant difference found. e Cell proliferation determined as percent Ki-67 positive cells of total count. No statistical difference observed. f Percentages of hypoxia in tumor from PIMO antibody staining. No statistical difference found