Figure 9

Example of application of the EFLCM method: To visualize thousands of cells growing in monolayer – for automatic and objective quantitation of epigenetic effects of the transformation associated viral nuclear antigen HHV8 LANA fused to green fluorescence protein in a transient transfection assay. The blue color is Hoecsht 33342 fluorescence showing DNA. The mosaic is built up from 100 images (10 columns × 10 rows) captured at 16X/NA O.5. Individual images covers an area of 522 μm × 398 μm. Images are captured at binning 1 (1344 × 1024 pixels) with 100 pixels overlap resulting in a final area of the seamless panorama, after image processing in the ImageLab/Virtual Microscope, of 4,82 mm × 3,58 mm.