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Figure 8 | BMC Medical Imaging

Figure 8

From: Extended Field Laser Confocal Microscopy (EFLCM): Combining automated Gigapixel image capture with in silicovirtual microscopy

Figure 8

Breaking the Gigapixel limit of a confluent confocal panorama image: A 2,8 Gigapixel size confocal mosaic, built up from 3000 (50 columns × 60 rows) individual confocal images (A), using the ImageLab/Virtual Microscope software. The internal structures of a mouse head are visualized with eosin fluorescence at 63X/NA1.25, individual images cover an area of 135 μm × 103 μm (B), captured at binning 1 (1344 × 1024 pixels) and 200 pixels overlap. After image processing in the ImageLab/Virtual Microscope software the final seamless confocal panorama show an area of 5,75 mm × 4,98 mm. The original resolution of each individual image is preserved in the final montage, allowing the user to zoom in on details at single cell level (C).

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BMC Medical Imaging

ISSN: 1471-2342

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