Figure 11

Example of a semi-confocal application of the EFLCM method: Using EFLCM we easily capture images of an entire slide, here showing Hoecsht 33342 fluorescence from DNA in all cells of a whole mouse fetus. The mosaic is built up from 782 images (17 columns × 46 rows) captured at 10X/NA O,25. Individual images covers an area of 835 μm × 636 μm. Images are captured at binning 2 (672 × 512 pixels) with 100 pixels overlap resulting in a final area, after image processing in the ImageLab/Virtual Microscope, of 12,1 mm × 23,6 mm.