Pathologist | Computer |
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A. Visually examines tissue | I. Captures digital image of relevant area of tissue |
B. Locates invasive carcinoma | Â |
C. Examines intensity of stain at cytoplasmic membrane | II. Identifies stained membrane areas (pixels) and quantifies intensity on 0–255 scale |
D. Examines completeness of membrane staining | III. Quantifies percentage of membrane staining per cell |
E. Examines percent positive cells | IV. Counts positive cancer cells and divides by total number of cancer cells |
F. Compares with positive tissue controls | V: Takes digital image of control, quantifies control, and uses it to normalize patient HER2 score. |