Figure 2

Quantification of percent adipose tissue in upper back/shoulder region. For a given sagittal mid-slice MRI image, both the total volume and the subcutaneous (sc) fat volumes were selected using the Connected Threshold Grower tool. Subcutaneous adipose tissue (sc) to be quantified was pre-determined by truncating the raw MR image with upper and lower black-colored lines which extend horizontally from the tip of the nose to the back of the neck and horizontally from the lowermost point of the twelfth spinal vertebra (illustrated by the yellow lines in the top image). The corresponding volumes were determined using the Voxel Counter tool. The percent subcutaneous adipose tissue was calculated for by dividing the voxel count determined for the sc fat by the total voxels for the slice.