Figure 1

Schematic of the proposed methodology. Proposed methodology is summarized in Figure 1. Stabilization of the videos is not included in the schematics. After stabilization, the weighted mean of five consecutive frames is calculated and the preprocessing and segmentation algorithms are applied on the mean frame. Averaging more than five frames results in “over-averaging” phenomena that would eliminate some of the important features important for segmentation. Experimentally, our results indicate that the 5-frame approach provides the best results. After the segmentation, the segmented frames are combined together to generate one single binary image and calculate Functional Capillary Density from this binary image.