Time course of PCA score plot. Graphs located on the left side represent PCA score plots of each group, and graphs on the right side represent PC loading plots corresponding to each graph on the left. Graphs indicate the data from rats sacrificed immediately after anesthesia (A), 24 h after completion of anesthesia (B), and 48 h after completion of anesthesia (C). Variables of each graph are (A) n = 31 reduced from 186, (B) n = 31 reduced from 186, (C) n = 31 reduced from 186. The different marks represent each group: propofol (white), dexmedetomidine (blue), sevoflurane (yellow), and isoflurane (purple). The PCA scores plot revealed that the differences between anesthetized groups decreased with time. The effect of propofol and dexmedetomidine resolved by the 24-h time-point; however, the effect of sevoflurane and isoflurane persisted (B).