Fig. 5

A and B Renal interstitial fibrosis (black star) on Masson’s trichrome staining of OS and IS (400 × magnification.); C and D NF-κB expression (black arrow) on immunostaining of OS and IS (400 × magnification.). E and F The MOD of Masson trichrome staining different group in different time points of OS and IS (400 × magnification.). (G-H) The MOD of NF-κB immunostaining of different group in different time points of OS and IS (400 × magnification.). * generated from comparisons among the four groups at each time point using one-way ANOVA. OS, outer stripe of outer medulla; IS, inner stripe of outer medulla; HUA, hyperuricemia; AP, allopurinol; AP + EM, allopurinol + empagliflozin; NF-κB, nuclear factor kappa B; MOD, mean optical density