Evaluation of living liver donors using contrast enhanced multidetector CT – The radiologists impact on donor selection
© Ringe et al.; licensee BioMed Central Ltd. 2012
Received: 12 March 2012
Accepted: 24 July 2012
Published: 24 July 2012
Living donor liver transplantation (LDLT) is a valuable and legitimate treatment for patients with end-stage liver disease. Computed tomography (CT) has proven to be an important tool in the process of donor evaluation. The purpose of this study was to evaluate the significance of CT in the donor selection process.
Between May 1999 and October 2010 170 candidate donors underwent biphasic CT. We retrospectively reviewed the results of the CT and liver volumetry, and assessed reasons for rejection.
89 candidates underwent partial liver resection (52.4%). Based on the results of liver CT and volumetry 22 candidates were excluded as donors (31% of the cases). Reasons included fatty liver (n = 9), vascular anatomical variants (n = 4), incidental finding of hemangioma and focal nodular hyperplasia (n = 1) and small (n = 5) or large for size (n = 5) graft volume.
CT based imaging of the liver in combination with dedicated software plays a key role in the process of evaluation of candidates for LDLT. It may account for up to 1/3 of the contraindications for LDLT.
KeywordsLDLT Transplantation Living donor Recipient CT
Since the first report of successful living donor liver transplantation (LDLT) in 1990 , LDLT has become a valuable treatment for patients with end-stage liver disease who cannot receive deceased donor livers. Particularly in children who need a small sized graft, the use of liver transplantation is limited due to shortage of deceased donor organs.
Since the safety of volunteer living donors in LDLT has always been considered paramount, evaluation of potential candidates plays a crucial role to confirm suitability and to identify possible contraindications. Each transplant center has its own protocol for living donor evaluation, which typically includes a comprehensive medical and psychosocial examination as well as non-invasive imaging and other studies to assess size, anatomy and function of the liver.
Both, contrast enhanced computed-tomography (CT), and magnetic resonance imaging (MRI) have been shown to be suitable diagnostic tools, and are being used concurrently in different centers throughout the world. Imaging in a living liver donor has three objectives: (1) to identify any intraparenchymal lesions or abnormalities like fatty changes; (2) to visualize the extra- and intrahepatic vascular and biliary anatomy; and (3) to determine the size of the whole liver and calculate the graft and remnant liver volumes. The main advantage of CT over MRI is based on a higher spatial resolution and manifold post-processing possibilities [2, 3].
The preoperative imaging process in LDLT is demanding and conscientious. Radiologists play a key role in filtering and providing the required information to surgeons. In addition, they may help to identify unsuitable donors and avoid unnecessary or invasive studies and procedures. The purpose of our study was thus to evaluate the significance of multidetector CT in this process, and therefore to reflect the radiologists impact on donor selection.
Materials and methods
This retrospective study was approved by the ethics committee of Hannover Medical School with a waiver of consent granted.
Candidate donors and recipients
Candidate donor demographic data
Number of potential donors
Donor age (years)
18-61 (mean 39)
Donor sex (male / female)
78 / 92
Mean graft volume [ml]
277 (SD 63)
Full left lobe
1134 (SD 317)
Recipient demographic data
Number of potential recipients
4 months - 71 years (mean 15.3 years)
Patient sex (male / female)
90 / 53
Tumour (HCC, Hepatoblastoma)
Acute liver failure
Until October 2005 CT was performed using a 4-channel multi-detector row CT (Somatom Plus 4A, Siemens, Erlangen, Germany). To keep radiation dose levels as low as possible, a native CT scan was not acquired. 150 ml of a nonionic iodinated contrast agent (Ultravist 300®, Bayer Schering Pharma, Berlin) followed by a 40 ml saline flush (NaCl 0.9%) were injected at a flow of 3-5 ml/sec. Biphasic image acquisition of the liver started 5 seconds after bolus detection in the abdominal aorta for the arterial phase. Portal-venous phase scanning followed after an interscan delay of 15 seconds. The parameters were identical for both scans: 3 mm slice collimation, a table feed of 5 mm per gantry rotation, and 2 mm reconstruction interval. Starting in October 2005 CT was performed using a 64-channel scanner (Lightspeed VCT, GE Medical Systems, USA). Multiphase scanning was performed after intravenous injection of 120 ml of a nonionic iodinated contrast medium (Imeron 400®, Nycomed GmbH, Germany) followed by a 50 ml saline flush (NaCl 0,9%) at a rate of 5 ml/sec. Arterial dominant phase images were obtained 15 seconds after bolus detection in the abdominal aorta, portal-venous scanning followed after an interscan delay of 25 seconds. Again, the parameters were identical for both scans: 1.25 mm slice collimation, a table feed of 39.37 mm per gantry rotation, and 1 mm reconstruction interval.
Image analysis and postprocessing
To meet the need of increasing potential liver transplant recipients, alternative procedures have been developed, such as reduced-size, split and living donor liver transplantation . LDLT has two major advantages over transplantation from a brain-dead donor: excellent graft quality and reduced ischemia-reperfusion injury .
On the other hand, donor safety is the first priority. It is important to keep in mind that LDLT should only be performed if the risk to the donor is justified by expectation of an acceptable outcome in the recipient. Overall donor morbidity is estimated to be approximately 35%, including bile leakage, wound infection and ileus . A recent survey identified 33 living liver donor deaths, including 3 donors who succumbed after an attempted rescue with a liver transplant .
Preoperative imaging of the liver is essential in order to identify and minimize the individual risk of the potential donor. It is further determining for graft survival and in preventing vascular complications. The implementation of imaging studies of the liver, the choice of the imaging modality (CT, MRI, ultrasound, angiography, e.g.) as well as the timing of imaging (early in the evaluation process vs. in the end) varies in different transplant centers.
MRI is undoubtedly becoming more significant in the course of living donor liver evaluation, due to the development of hepatobiliary-specific contrast agents and new imaging techniques (such as chemical shift imaging, MR spectroscopy), opening up new possibilities for comprehensive imaging of the liver [12, 13]. However, availability of MR is still an issue.
The purpose of this retrospective study was therefore to review the radiologist’s contribution to the process of evaluation of donors for LDLT by means of contrast enhanced CT, especially since we can look back on a large group of donors being evaluated with this technique over a significant period of time. Based on the results of the CT scan and liver volumetry in our study 22 candidates were excluded as donors, which accounts for nearly one third (31%) of the cases in which LDLT was not carried out. This relatively high number could be an argument to perform CT imaging early rather than late in the process of donor evaluation. The overall costs for complete donor evaluation have been estimated in previous studies to be in the range from 1383-2569€ , in some centers even as high as 4589€ . Early implementation of CT imaging (estimated costs of approximately 400-600€) in the evaluation process might therefore prevent further studies in unsuitable donors and reduce costs.
Nine candidate donors were excluded because CT indicated steatosis of the liver, which was confirmed with ultrasound and liver biopsy. Fatty infiltration in hepatic grafts is known to be an important risk factor for primary graft nonfunction in deceased donor liver transplantation as well as in LDLT [16, 17]. Liver grafts with a mild degree of fatty changes can be used for liver transplantation without ill effect, but liver grafts with moderate or severe degree of fatty changes have been found to have a negative effect on post transplant graft function and patient survival [17, 18]. Marcos et al estimated that 1% of hepatic steatosis can decrease the functional graft mass by 1% . More recently the same group published a series in which no impairment in function was found in either the living donor or the recipient using grafts containing less than 30% steatosis . In our own institution the acceptable upper limit of fatty changes in the liver graft is 30%.
10 candidate donors were excluded because of the results of liver volumetry, depicting either small (n = 5) or large for size (n = 5) graft volume. Preoperative assessment of total, graft and remnant liver volume is of utmost importance, since inadequate liver mass can influence patient and graft survival. There are several formulas for calculating total liver volume depending on the body weight, body surface and gender [21, 22]. Results do not always correlate and liver volume is often overestimated . Depending on the individual method used, it is considered acceptable when the ratio between graft weight and recipient body weight or between graft volume and the estimated standard liver volume of the recipient are at least 0,8% and 40%, respectively .
In case of left lateral donation remnant liver volume is usually safe for the donor, whereas graft size might be too large in small children. In our study this led to the exclusion of five donors. Especially in case of right liver donation remnant liver volume might be too small (risking acute liver failure of the donor), whereas graft size might as well be too small resulting in a “small-for-size-syndrome” in the recipient, characterized by hepatocyte ballooning, steatosis, centrilobular necrosis and parenchymal cholestasis . This led to the exclusion of three donors who were evaluated for donation of the right lobe.
CT is further important in assessment of vascular hepatic anatomy. Certain anomalies may require modification of the surgical procedure, while others might be a contraindication for surgery . Other vascular variants may even be advantageous, e.g. a displaced right artery arising from the superior mesenteric artery in case of right donation. Due to the greater variability of the right hepatic vascular anatomy right hepatectomy can be one of the most challenging surgical procedures. In addition to the arterial supply of the graft and the remnant liver attention has to be paid to the venous drainage in order to prevent venous congestion. In our study 4 candidate donors were excluded from LDLT because of various vascular anatomic variants. Even though our reported vascular variants do not present absolute contraindications, in these specific cases LDLT would have been involved with an increased surgical complexity and an increased risk of graft failure respectively complications in the donor as well as in the recipient. It is important that anatomic vascular variants are assessed in context with the planned resection, and the decision whether a specific donor is suited for a specific recipient is in the end up to the responsible transplant surgeon.
The use of imaging studies in the process of liver donor evaluation, specifically the choice of the imaging modality (CT, MRI, ultrasound, or angiography) as well as the timing of imaging (early in the evaluation process vs. in the end) has significant implications for the subsequent evaluation process. In our long-term experience, CT based imaging of the liver in combination with dedicated software plays a key role in the process of evaluation of candidates for LDLT. In this series almost 1/3 of donor candidates were rejected because of CT findings. CT can help to reduce the risk for donor and recipient by exclusion of unsuitable donor livers. If performed early during the evaluation process it can also prevent unnecessary studies, further reducing the risks and costs.
- Strong RW, Lynch SV, Ong TH, Matsunami H, Koido Y, Balderson GA: Successful liver transplantation from a living donor to her son. N Engl J Med. 1990, 322: 1505-1507. 10.1056/NEJM199005243222106.View ArticlePubMedGoogle Scholar
- Prokop M, Shin H, Schanz A, Schaefer-Prokop CM: Use of maximum intensity projections in CT angiography: a basic review. Radiographics. 1997, 17: 433-451.View ArticlePubMedGoogle Scholar
- Sahani D, Saini S, Pena C, Nichols S, Prasad SR, Hahn PF, Halpern EF, Tanabe KK, Mueller PR: Using multidetector CT for preoperative vascular evaluation of liver neoplasms: technique and results. AJR Am J Roentgenol. 2002, 179: 53-59.View ArticlePubMedGoogle Scholar
- Johnston RJ, Stamm ER, Lewin , Hendrick RE, Archer PG: Diagnosis of fatty infiltration of the liver on contrast enhanced CT: limitations of liver-minus-spleen attenuation difference measurements. Abdom Imaging. 1998, 23: 409-415. 10.1007/s002619900370.View ArticlePubMedGoogle Scholar
- Panicek DM, Giess CS, Schwartz LH: Qualitative assessment of liver for fatty infiltration on contrast-enhanced CT: is muscle a better standard of reference than spleen?. J Comput Assist Tomogr. 1997, 21: 699-705. 10.1097/00004728-199709000-00004.View ArticlePubMedGoogle Scholar
- Frericks BB, Caldarone FC, Nashan B, Savellano D, Stamm G, Kirchhoff TD, Shin HO, Schenk A, Selle D, Spindler W, Klempnauer J, Peitgen HO, Galanski M: 3D CT modeling of hepatic vessel architecture and volume calculation in living donated liver transplantation. Eur Radiol. 2004, 14: 326-333. 10.1007/s00330-003-2161-8.View ArticlePubMedGoogle Scholar
- Frericks BB, Kirchhoff TD, Shin HO, Stamm G, Merkesdal S, Abe T, Schenk A, Peitgen HD, Klempnauer J, Galanski M, Nashan B: Preoperative volume calculation of the hepatic venous draining areas with multi-detector row CT in adult living donor liver transplantation: Impact on surgical procedure. Eur Radiol. 2006, 16: 2803-2810. 10.1007/s00330-006-0274-6.View ArticlePubMedGoogle Scholar
- Alonso-Torres A, Fernandez-Cuadrado J, Pinilla I, Parron M, de Vicente E, Lopez-Santamaria M: Multidetector CT in the evaluation of potential living donors for liver transplantation. Radiographics. 2005, 25: 1017-1030. 10.1148/rg.254045032.View ArticlePubMedGoogle Scholar
- Kawasaki S, Makuuchi M, Matsunami H, Hashikura Y, Ikegami T, Nakazawa Y, Chisura H, Terada M, Miyagawa S: Living related liver transplantation in adults. Ann Surg. 1998, 27: 269-274.View ArticleGoogle Scholar
- Barr LM, Belghiti J, Villamil FG, Pomfret EA, Sutherland DS, Gruessner RW, Langnas AN, Delmonico FL: A report of the Vancouver forum on the care of the live organ donor: lung, liver, pancreas, and intestine data and medical guidelines. Transplantation. 2006, 81: 1373-1385. 10.1097/01.tp.0000216825.56841.cd.View ArticlePubMedGoogle Scholar
- Ringe B, Strong RW: The dilemna of living liver donor deaths: to report or not to report?. Transplantation. 2008, 85: 790-10.1097/TP.0b013e318167345e.View ArticlePubMedGoogle Scholar
- Ma X, Holalkere NS, Kambadakone RA, Mino-Kenudson M, Hahn PF, Sahani DV: Imaging-based quantification of hepatic fat: methods and clinical applications. Radiographics. 2009, 29: 1253-1277. 10.1148/rg.295085186.View ArticlePubMedGoogle Scholar
- Seale MK, Catalano OA, Saomo S, Hahn PF, Sahani DV: Hepatobiliary-specific MR contrast agents: role in imaging of the liver and biliary tree. Radiographics. 2009, 29: 1725-1748. 10.1148/rg.296095515.View ArticlePubMedGoogle Scholar
- Sagmeister M, Mullhaupt B, Kadry Z, Kullak-Unlick GA, Clavien PA, Renner EL: Cost-effectiveness of cadaveric and living-donor liver transplantation. Transplantation. 2002, 73: 616-622. 10.1097/00007890-200202270-00025.View ArticlePubMedGoogle Scholar
- Valentin-Gamazo C, Malago M, Karliova M, Lutz JT, Frilling A, Nadalin S, Testa G, Ruehm SG, Erim Y, Paul A, Lang H, Gerken G, Broelsch CE: Experience after the evaluation of 700 potential donors for living donor liver transplantation in a single center. Liver Transpl. 2004, 10: 1087-1096. 10.1002/lt.20223.View ArticlePubMedGoogle Scholar
- Todo S, Demetris AJ, Makowka L, Teperman L, Podesta L, Shaver T, Tzakis A, Starzl TE: Primary nonfunction of hepatic allografts with preexisting fatty infiltration. Transplantation. 1989, 47: 903-905. 10.1097/00007890-198905000-00034.View ArticlePubMedPubMed CentralGoogle Scholar
- D'Alessandro AM, Kalayoglu M, Sollinger HW, Hoffmann RM, Reed A, Knechtle SJ, Pirsch JD, Hafez GR, Lorentzen D, Belzer FO: The predictive value of donor liver biopsies for the development of primary nonfunction after orthotopic liver transplantation. Transplantation. 1991, 51: 157-163. 10.1097/00007890-199101000-00024.View ArticlePubMedGoogle Scholar
- Moon D, Lee S, Hwang S, Kim K, Ahn C, Park K, Ha T, Song G: Resolution of severe graft steatosis following dual-graft living donor liver transplantation. Liver Transpl. 2006, 12: 1156-1160. 10.1002/lt.20814.View ArticlePubMedGoogle Scholar
- Marcos A, Ham JM, Fisher RA, Fisher RA, Olzinski AT, Posner MP: Single-center analysis of the first 40 adult-to-adult living donor liver transplants using the right lobe. Liver Transpl. 2000, 6: 296-301. 10.1053/lv.2000.6354.View ArticlePubMedGoogle Scholar
- Marcos A, Fisher RA, Ham JM, Shiffman ML, Sanyal AJ, Luketic VA, Sterling RK, Fulcher AS, Posner MP: Liver regeneration and function in donor and recipient after right lobe adult to adult living donor liver transplantation. Transplantation. 2000, 69: 1375-10.1097/00007890-200004150-00028.View ArticlePubMedGoogle Scholar
- Urata K, Kawasaki S, Matsunami H, Hasikura Y, Ikegami T, Ishizone S, Momose Y, Komiyama A, Makuchi M: Calculation of child and adult standard liver volume for liver transplantation. Hepatology. 1995, 21: 1317-1321. 10.1002/hep.1840210515.View ArticlePubMedGoogle Scholar
- DeLand FH, North WA: Relationship between liver size and body size. Radiology. 1968, 91: 11951-1198.View ArticleGoogle Scholar
- Kamel IR, Kruskal JB, Warmbrand G, Goldberg SN, Pomfret EA, Raptopoulos V: Accuracy of volumetric measurements after virtual right hepatectomy in potential donors undergoing living adult liver transplantation. Am J Roentegnol AJR. 2001, 176: 483-487.View ArticlePubMedGoogle Scholar
- Chen YS, Cheng YF, De Villa VH, De Villa VH, Wang CC, Lin CC, Huang TL, Jawan B, Chen CL: Evaluation of living liver donors. Transplantation. 2003, 75 (3 Suppl): S16-19.View ArticlePubMedGoogle Scholar
- Emond JC, Renz JF, Ferrell LD, Rosenthal P, Lim RC, Roberts JP, Lake JR, Ascher NL: Functional analysis of grafts from living donors. Implications for the treatment of older recipients. Ann Surg. 1996, 224: 544-552. 10.1097/00000658-199610000-00012.View ArticlePubMedPubMed CentralGoogle Scholar
- Catalano OA, Singh AH, Uppot RN, Hahn PF, Ferrone , Sahani DV: Vascular and biliary variants in the liver: implications for liver surgery. Radiographics. 2008, 28: 359-378. 10.1148/rg.282075099.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2342/12/21/prepub