Unusual MRI findings in an immunocompetent patient with EBV encephalitis: a case report
© Di Carlo et al; licensee BioMed Central Ltd. 2011
Received: 10 September 2010
Accepted: 24 March 2011
Published: 24 March 2011
It is well-known that Epstein-Barr virus (EBV) can affect the central nervous system (CNS).
Herein the authors report unusual timely Magnetic Resonance Imaging (MRI) brain scan findings in an immunocompetent patient with EBV encephalitis.
Diffusion weighted MRI sequence performed during the acute phase of the disease was normal, whereas the Fast Relaxation Fast Spin Echo T2 image showed diffuse signal intensity changes in white matter. The enhancement pattern suggested an inflammatory response restricted to the brain microcirculation. Acyclovir and corticosteroid therapy was administered. After three weeks, all signal intensities returned to normal and the patient showed clinical recovery.
This report demonstrates that EBV in an immunocompetent adult can present with diffuse, reversible brain white matter involvement in the acute phase of mononucleosis. Moreover, our case suggests that a negative DWI sequence is associated with a favorable improvement in severe EBV CNS infection. More extensive studies are needed to assess what other instrumental data can help to distinguish viral lesions from other causes in the acute phase of disease.
KeywordsEpstein-Barr virus encephalitis MRI Diffusion-weighted imaging
Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis (IM) and can lead to various central nervous system (CNS) complications, such as demyelinating disease, acute encephalitis, meningitis and acute cerebellar ataxia [1–3]. The illness usually runs a benign course, though fatal cases have been reported .
Neurological involvement has been reported in children during the acute phase of the disease and sequelae occur in a substantial number of patients [4–6]. Neurological manifestations associated with reactivation of EBV infection in immunocompetent adults have recently been reported [7, 8]. Furthermore, evidence suggests that EBV plays a role in multiple sclerosis (MS) disease activity and in Acute disseminate encephalomyelitis (ADEM), an immune-mediated inflammatory disorder of the CNS, characterized by a multifocal demyelination that involves the white matter of the brain and spinal cord [9, 10].
Magnetic resonance imaging (MRI) shows small or multiple CNS lesions more clearly, allowing rapid diagnosis and the formulation of more effective therapeutic strategies [6, 11]. Diffusion-weighted imaging (DWI) sequence identifies CNS lesions earlier than T2W or Fluid Attenuated Inversion Recovery (FLAIR) imaging [11–15].
Herein we report an unusual timely CNS imaging sequence in an immunocompetent patient with acute infectious mononucleosis.
A 19-year-old Italian male student came to the Emergency Department of the "Policlinico Paolo Giaccone" University Hospital in Palermo, Italy, with fever, headache and altered consciousness. The fever started one week before presentation; he had experienced pharyngitis, lymphadenopathy and asthenia. He had no other history of disease and was immunocompetent. An initial cranial CT scan was normal.
On admission, he had a Glasgow score of 12 and a temperature of 38.6°C; he had visual disorders including blurred vision, impaired accommodation and diplopia.
He presented with cervical lymphadenopathy and hepatosplenomegaly.
Laboratory tests revealed that he had a white blood cell count of 15400 cell mm-3, with 20% neutrophils, 36,2% lymphocytes, 33,1% monocytes and hypertransaminasemia (aspartate aminotransferase level of 78 IU l-1 and an alanine aminotransferase level of 271 IU l-1).
Lumbar puncture revealed 81 cells/μL with 27% mononuclear cells and a protein content of 102 mg dl-1.
Real Time quantitative PCR of Cerebrospinal Fluid (CSF) was positive for EBV (2.200 copies/mL). We used the BAMHI-W fragment region of the EBV genome as the target of our PCR screening .
PCR of the CSF sample was negative for Herpes Virus 1 And 2, Varicella Zoster Virus, Cytomegalovirus and Enterovirus. Bacterial cultures were negative for Mycobacterium tuberculosis and other pathogens which are epidemiologically relevant in our geographic area (e.i. Rickettsia conorii, Brucella spp).
On admission, serological tests for HIV, VDRL, CMV, HSV, hepatitis A, hepatitis C, b Borrelia and Brucella were all negative. Serological tests for EBV confirmed primary infection: VCA IgM positive, EA IgG positive, VCA IgG positive, and EBNA IgG negative.
An MRI scan of the brain using gadolinium contrast enhancement was performed 18 hours after the patient was admitted to hospital. Images were taken of the axial, coronal and sagittal sections.
MRI sequence parameters.
b, s/mm 2
256 × 256
128 × 128
256 × 256
256 × 256
Fourth ventricle size was reduced, which is consistent with cerebral edema, and we also observed hyperintensity in the ponto-mesencephalic region (cortico spinal tract), on the T2 FLAIR sequence. (figure 1, C).
Contrast agent enhancement pattern suggested an inflammatory response restricted to the brain microcirculation (figure 1, D).
The patient was treated with intravenous acyclovir (10 mg/kg every 8 h) combined with methylprednisolone (500 mg intravenous bolus for 3 days then 1 mg/kg daily for 21 days).
Neurological improvement was noted at day 10 (Glasgow score of 14) but his diplopia symptoms persisted. PCR on CSF performed three weeks after admission was negative for EBV.
Scanner and sequence parameters were identical to those used previously (Table 1).
Six months later, the patient had residual diplopia but was otherwise normal.
A brain infection is a medical emergency. Immediate diagnosis and initiation of symptomatic and specific therapy has a dramatic influence upon survival and reduces the extent of permanent brain damage in survivors. In cases of herpes virus encephalitis, EBV has rarely been reported as the cause of CNS lesions, although fatal events and severe sequelae have been described, mostly in cases of acute primary infection [17, 18].
DWI sequences provide image contrast that is dependent on the molecular motion of water; this is important in cases of increased or restricted diffusion, such as ischemic stroke, intracranial infections or trauma[6, 11–13]. Viral infections of the nervous system are characterized by alterations in water diffusion caused by cytotoxic edema, which can be detected by DWI. Reports have shown that DWI is usually the first sequence that is activated during cytotoxic cortical edema in tissue undergoing necrosis in EBV encephalitis [11–15].
In our case, we did not detect any abnormal signs in the DWI sequence, whereas T2W images showed mainly sub- and supra-tentorial white matter demyelination.
Our young patient's favorable course of CNS EBV infection and clearance suggests that EBV damage may be reversible when no abnormalities are detected on DWI, and appropriate therapy is initiated .
Apparent Diffusion Coefficient (ADC) maps can predict outcome in acute encephalitis ; we did not calculate this at the time of admission as this feature is not available at our hospital under emergency conditions.
We observed diffuse cerebral micro circular enhancement on T1W images after contrast agent was administered. In the course of inflammatory neurological disorders, adhesion molecule expression on endothelial cells is increased. This amplifies the inflammatory process in viral encephalitis where there is a lymphocytic infiltration of the CNS . Research studies conducted on animals suggest that the herpes virus has an endotheliotropism that may depend on the susceptibility of CNS microvascular endothelial cells . Moreover, EBV infection of human brain microvessel endothelial cells has recently been associated with the onset of multiple sclerosis .
Besides other infectious agents are responsible for mononucleosis-like illnesses, such as cytomegalovirus, HIV, cat scratch disease and syphilis, we investigated additional pathogens which are responsible for meningo-encephalitis in our geographic area, such as tuberculosis, rickettsial and brucellosis diseases.
ADEM was one of the non-infective CNS inflammatory diseases which we considered in the differential diagnosis of our patient. ADEM is a monophasic autoimmune demyelinating disease of the CNS that may occur after an infection or vaccination. However, ADEM lesions involve basal ganglia, thalami and the brainstem in particular  and this was not the case for our patient. Furthermore, clinical and instrumental resolution does not occur as early as it did in this case.
EBV-specific antiviral therapy does not exist, but acyclovir or gancyclovir have been recommended for the treatment of CNS involvement [6, 7]. There is some debate surrounding corticosteroid therapy, but it seems to be a reasonable option when vasculitis is suspected, as our patient's neuroimaging findings suggested.
In conclusion, this report demonstrates that EBV in an immunocompetent adult can present with diffuse, reversible brain white matter involvement in the acute phase of mononucleosis.
Written informed consent was obtained from the patient for publication of this case report and any accompanying images.
List of abbreviations
Apparent Diffusion Coefficient
Acute Disseminated Encephalomyelitis
Central Nervous System
Diffusion Weighted Imaging
Epstein-Barr virus nuclear antigen
Fluid Attenuated Inversion Recovery
Fast Relaxation Fast Spin Echo
Fast Spin Echo
Magnetic Resonance Imaging
Polymerase Chain Reaction
Viral Capsid Antigen
Venereal Disease Research Laboratory test
Human immunodeficiency virus
Herpes Simplex Virus.
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